Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10287 | 5' | -63 | NC_002687.1 | + | 16454 | 0.66 | 0.827232 |
Target: 5'- --aGuGCGCUuaUGGUCGaUCgCCCCUGa -3' miRNA: 3'- agaCuCGCGGcgACCAGC-AG-GGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 72298 | 0.66 | 0.779136 |
Target: 5'- aUCUccGGCGgCGUacGUCGUUCCCCCGa -3' miRNA: 3'- -AGAc-UCGCgGCGacCAGCAGGGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 42253 | 0.67 | 0.768158 |
Target: 5'- --aGAGCGCUGCUGcGacuucaggaggaucUUGUUCCCUCGa -3' miRNA: 3'- agaCUCGCGGCGAC-C--------------AGCAGGGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 173639 | 0.67 | 0.744842 |
Target: 5'- --cGAG-GCUGCUGG-CGUCCCUUCu -3' miRNA: 3'- agaCUCgCGGCGACCaGCAGGGGGGc -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 226040 | 0.67 | 0.744842 |
Target: 5'- cCUGAGUaGCCGuCUGuUCGUCCCggccgCCCa -3' miRNA: 3'- aGACUCG-CGGC-GACcAGCAGGG-----GGGc -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 240056 | 0.67 | 0.736051 |
Target: 5'- gUCUGgcGGCGUCuggaccguGCUGGUCGUacaCCCgCCGc -3' miRNA: 3'- -AGAC--UCGCGG--------CGACCAGCAg--GGG-GGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 13881 | 0.68 | 0.718254 |
Target: 5'- --aGAGCGCUcaUGGUCGaUCgCCCCUGa -3' miRNA: 3'- agaCUCGCGGcgACCAGC-AG-GGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 14739 | 0.68 | 0.718254 |
Target: 5'- --aGAGCGCUcaUGGUCGaUCgCCCCUGa -3' miRNA: 3'- agaCUCGCGGcgACCAGC-AG-GGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 15597 | 0.68 | 0.718254 |
Target: 5'- --aGAGCGCUcaUGGUCGaUCgCCCCUGa -3' miRNA: 3'- agaCUCGCGGcgACCAGC-AG-GGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 77627 | 0.68 | 0.700217 |
Target: 5'- cCUGuaauCGgCGCaaGUCGUCCCCCCa -3' miRNA: 3'- aGACuc--GCgGCGacCAGCAGGGGGGc -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 47165 | 0.69 | 0.635959 |
Target: 5'- gCUGGccggcacaucuGCGCCGCccgaGGcUGUCCCCCCu -3' miRNA: 3'- aGACU-----------CGCGGCGa---CCaGCAGGGGGGc -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 5883 | 1.07 | 0.002315 |
Target: 5'- gUCUGAGCGCCGCUGGUCGUCCCCCCGc -3' miRNA: 3'- -AGACUCGCGGCGACCAGCAGGGGGGC- -5' |
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10287 | 5' | -63 | NC_002687.1 | + | 6707 | 1.07 | 0.002315 |
Target: 5'- gUCUGAGCGCCGCUGGUCGUCCCCCCGc -3' miRNA: 3'- -AGACUCGCGGCGACCAGCAGGGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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