Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10288 | 5' | -54.2 | NC_002687.1 | + | 63493 | 0.66 | 0.99439 |
Target: 5'- uGUCCUCGaGgCCGaagUUAGCGGCccuGGCa -3' miRNA: 3'- -CAGGAGCaUgGGCca-AAUCGUCG---CUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 32545 | 0.66 | 0.991619 |
Target: 5'- uUUCUCGUAUCUGGaagaaAGCgAGUGACa -3' miRNA: 3'- cAGGAGCAUGGGCCaaa--UCG-UCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 156869 | 0.66 | 0.990487 |
Target: 5'- --aCUCGUGCCgGGUgguguccaUAGCAuggguccugcGCGACg -3' miRNA: 3'- cagGAGCAUGGgCCAa-------AUCGU----------CGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 91615 | 0.67 | 0.987863 |
Target: 5'- cUCUUCGggaaacuuuuCCCGGUagAGC-GCGACg -3' miRNA: 3'- cAGGAGCau--------GGGCCAaaUCGuCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 329781 | 0.67 | 0.982921 |
Target: 5'- -gCCgaggCGUGCCCGGU--GGC-GUGGCc -3' miRNA: 3'- caGGa---GCAUGGGCCAaaUCGuCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 160331 | 0.68 | 0.978868 |
Target: 5'- uUCCUUGUccuuaCUGGccUUGGCGGCGGCu -3' miRNA: 3'- cAGGAGCAug---GGCCa-AAUCGUCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 138655 | 0.68 | 0.974143 |
Target: 5'- aGUCCUUGcGCCCGGUcgaacUAGUGGauuaGGCg -3' miRNA: 3'- -CAGGAGCaUGGGCCAa----AUCGUCg---CUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 159126 | 0.68 | 0.968694 |
Target: 5'- -aCCUCG-ACCCGGagugGGCGuGUGACc -3' miRNA: 3'- caGGAGCaUGGGCCaaa-UCGU-CGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 170722 | 0.69 | 0.955442 |
Target: 5'- --gUUCGUGcCCCGGUUcgugggccgggUGGCAGCGGg -3' miRNA: 3'- cagGAGCAU-GGGCCAA-----------AUCGUCGCUg -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 280952 | 0.69 | 0.951611 |
Target: 5'- cGUCggCGgggGCCUGGguggcGGCAGCGGCg -3' miRNA: 3'- -CAGgaGCa--UGGGCCaaa--UCGUCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 111829 | 0.71 | 0.913076 |
Target: 5'- cGUCCUCGUuuucagagaCCGGUgucAGCacggGGCGACa -3' miRNA: 3'- -CAGGAGCAug-------GGCCAaa-UCG----UCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 146388 | 0.71 | 0.913076 |
Target: 5'- uGUCCUUGUGuCCgaCGGagaAGCGGCGGCg -3' miRNA: 3'- -CAGGAGCAU-GG--GCCaaaUCGUCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 175827 | 0.72 | 0.881833 |
Target: 5'- aUCCUCGUGUCCGGggUGGCAuGgGAa -3' miRNA: 3'- cAGGAGCAUGGGCCaaAUCGU-CgCUg -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 264358 | 0.74 | 0.778213 |
Target: 5'- aGUCCUCcUACaCCGGggcucguaAGCGGCGACu -3' miRNA: 3'- -CAGGAGcAUG-GGCCaaa-----UCGUCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 76614 | 0.78 | 0.59502 |
Target: 5'- -gCCUCGUGCUagaCGGU--GGCGGCGACg -3' miRNA: 3'- caGGAGCAUGG---GCCAaaUCGUCGCUG- -5' |
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10288 | 5' | -54.2 | NC_002687.1 | + | 10346 | 1.1 | 0.007956 |
Target: 5'- uGUCCUCGUACCCGGUUUAGCAGCGACg -3' miRNA: 3'- -CAGGAGCAUGGGCCAAAUCGUCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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