Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10289 | 5' | -51.2 | NC_002687.1 | + | 240659 | 0.66 | 0.999531 |
Target: 5'- --gGC-CCuUUCUCGUGaaaGCgGUGGAu -3' miRNA: 3'- aaaUGaGGuAAGAGCACg--UGgCACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 326935 | 0.66 | 0.999292 |
Target: 5'- --cGCcgCCGUUgUCGUGCA-CGUGGu -3' miRNA: 3'- aaaUGa-GGUAAgAGCACGUgGCACCu -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 207888 | 0.66 | 0.999292 |
Target: 5'- --gACaaCAUUCUCGUGC-CCGauggUGGAc -3' miRNA: 3'- aaaUGagGUAAGAGCACGuGGC----ACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 80499 | 0.66 | 0.99874 |
Target: 5'- -gUugUCCGaaucgUCUUGUGCGCUGaUGGu -3' miRNA: 3'- aaAugAGGUa----AGAGCACGUGGC-ACCu -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 207007 | 0.67 | 0.998196 |
Target: 5'- --gGgUCCuuUUCgggCGUGC-CCGUGGAg -3' miRNA: 3'- aaaUgAGGu-AAGa--GCACGuGGCACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 213704 | 0.67 | 0.997857 |
Target: 5'- -gUGCUCCuc-CUCGUuCACCGUGa- -3' miRNA: 3'- aaAUGAGGuaaGAGCAcGUGGCACcu -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 180712 | 0.68 | 0.995265 |
Target: 5'- --aACUCCAgca-CGUugaacGCGCCGUGGAc -3' miRNA: 3'- aaaUGAGGUaagaGCA-----CGUGGCACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 159323 | 0.7 | 0.980747 |
Target: 5'- --aACUCCGUUgUCGgGCG-CGUGGAa -3' miRNA: 3'- aaaUGAGGUAAgAGCaCGUgGCACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 259617 | 0.74 | 0.915393 |
Target: 5'- cUUGC-CCGaucCUCGUGgGCCGUGGAa -3' miRNA: 3'- aAAUGaGGUaa-GAGCACgUGGCACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 320493 | 0.74 | 0.903382 |
Target: 5'- --aACUCUAUUgUUGUGCACC-UGGAg -3' miRNA: 3'- aaaUGAGGUAAgAGCACGUGGcACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 245423 | 0.74 | 0.903382 |
Target: 5'- --aACUCUAUUgUUGUGCACC-UGGAg -3' miRNA: 3'- aaaUGAGGUAAgAGCACGUGGcACCU- -5' |
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10289 | 5' | -51.2 | NC_002687.1 | + | 10939 | 1.07 | 0.020541 |
Target: 5'- uUUUACUCCAUUCUCGUGCACCGUGGAa -3' miRNA: 3'- -AAAUGAGGUAAGAGCACGUGGCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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