Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1029 | 5' | -57.4 | NC_000924.1 | + | 38791 | 0.66 | 0.5658 |
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Target: 5'- gGGCCUGCUggUCCGC-GUGGUgagAAGgGCg -3' miRNA: 3'- aUUGGACGA--GGGUGuCGCCG---UUCgCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 30030 | 0.66 | 0.555035 |
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Target: 5'- -cGCCUGCauaUCUCAgAGCacGGCAAaauuuGCGCg -3' miRNA: 3'- auUGGACG---AGGGUgUCG--CCGUU-----CGCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 24744 | 0.67 | 0.491959 |
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Target: 5'- cAGCCagcGCUCCCGaaaccuuaaAGCGGCA-GCGa -3' miRNA: 3'- aUUGGa--CGAGGGUg--------UCGCCGUuCGCg -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 14950 | 0.68 | 0.481758 |
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Target: 5'- cAACCUGUaCCagaACAuGCGGCcaauGCGCu -3' miRNA: 3'- aUUGGACGaGGg--UGU-CGCCGuu--CGCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 37002 | 0.69 | 0.38611 |
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Target: 5'- -uGCCUGUUCUCcCGGUgaGGUGAGCGUg -3' miRNA: 3'- auUGGACGAGGGuGUCG--CCGUUCGCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 59782 | 0.7 | 0.377253 |
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Target: 5'- cUGugCUGCUCCCAgcuccacucuCAGCuucccaaccGUAAGCGCa -3' miRNA: 3'- -AUugGACGAGGGU----------GUCGc--------CGUUCGCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 7244 | 0.71 | 0.319176 |
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Target: 5'- cAGCCaGCuUCCCAgcCAGCGuuGCGAGUGCu -3' miRNA: 3'- aUUGGaCG-AGGGU--GUCGC--CGUUCGCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 41573 | 0.71 | 0.303113 |
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Target: 5'- aGGCCUGUcauauucUCUgGCGGCGGCAGGUa- -3' miRNA: 3'- aUUGGACG-------AGGgUGUCGCCGUUCGcg -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 46829 | 0.73 | 0.229706 |
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Target: 5'- uUGGCgCUGgUCUgGguGCGGCAGGUGCa -3' miRNA: 3'- -AUUG-GACgAGGgUguCGCCGUUCGCG- -5' |
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| 1029 | 5' | -57.4 | NC_000924.1 | + | 56442 | 1.1 | 0.000431 |
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Target: 5'- aUAACCUGCUCCCACAGCGGCAAGCGCg -3' miRNA: 3'- -AUUGGACGAGGGUGUCGCCGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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