miRNA: 3'- -GGAu-UUAUUUGUU--GUGAG---GCCAGACU- -5'
miRNA: 3'- gGAUUuaUUUGUUGuGAGGCCAgacu -5'
miRNA: 3'- ggaUUUaUUUgUUGUGAGGCCAGacu -5'
miRNA: 3'- -GGAUUUAuu---UGUUGUGaGGCCAGACU- -5'
miRNA: 3'- -GGauuuauuUGUUGUGAGgCCAGACU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10291 | 3' | -43.8 | NC_002687.1 | + | 87747 | 0.66 | 1 |
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Target: 5'- gCCUcuGGUAGACGGgcUGCUCaagCGGUCUGGg -3' miRNA: 3'- -GGAu-UUAUUUGUU--GUGAG---GCCAGACU- -5' |
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| 10291 | 3' | -43.8 | NC_002687.1 | + | 48145 | 0.68 | 1 |
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Target: 5'- aCUGGggGGACAGC-CUCCGGUg--- -3' miRNA: 3'- gGAUUuaUUUGUUGuGAGGCCAgacu -5' |
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| 10291 | 3' | -43.8 | NC_002687.1 | + | 283022 | 0.68 | 1 |
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Target: 5'- --gAAAaAAAgAGCACUCCGGUCc-- -3' miRNA: 3'- ggaUUUaUUUgUUGUGAGGCCAGacu -5' |
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| 10291 | 3' | -43.8 | NC_002687.1 | + | 147477 | 0.67 | 1 |
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Target: 5'- uCUUGGAUugaacGCGAUGC-CUGGUCUGAc -3' miRNA: 3'- -GGAUUUAuu---UGUUGUGaGGCCAGACU- -5' |
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| 10291 | 3' | -43.8 | NC_002687.1 | + | 281354 | 0.66 | 1 |
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Target: 5'- uCCggu----ACAACACUCaGGUUUGAu -3' miRNA: 3'- -GGauuuauuUGUUGUGAGgCCAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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