Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10292 | 3' | -51.7 | NC_002687.1 | + | 87708 | 0.68 | 0.992139 |
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Target: 5'- cGCCG-GGCGUgUGGCGCUGACc---- -3' miRNA: 3'- -CGGCgUUGCGaACCGUGACUGuaagu -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 135619 | 0.69 | 0.989817 |
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Target: 5'- aCCGCGACGCggugcagcgguUUGGCAacggggacauCUGGCAggCAu -3' miRNA: 3'- cGGCGUUGCG-----------AACCGU----------GACUGUaaGU- -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 44252 | 0.69 | 0.98699 |
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Target: 5'- cUCGCAGCGUUuuuuaUGGaCGCUGGC-UUCAu -3' miRNA: 3'- cGGCGUUGCGA-----ACC-GUGACUGuAAGU- -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 309927 | 0.71 | 0.956009 |
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Target: 5'- gGuuGCAGCGCgggcGGCACcgccGACGUUCc -3' miRNA: 3'- -CggCGUUGCGaa--CCGUGa---CUGUAAGu -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 26403 | 0.73 | 0.906903 |
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Target: 5'- --gGCu-CGCUUGGCACUGGCAUa-- -3' miRNA: 3'- cggCGuuGCGAACCGUGACUGUAagu -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 17888 | 0.74 | 0.88769 |
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Target: 5'- gGCCGCAACGCUUGG-AgUGuACAUcCAc -3' miRNA: 3'- -CGGCGUUGCGAACCgUgAC-UGUAaGU- -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 17999 | 0.82 | 0.498703 |
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Target: 5'- gGCCGCAACGCUUGGaGCgGACAUcCAc -3' miRNA: 3'- -CGGCGUUGCGAACCgUGaCUGUAaGU- -5' |
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| 10292 | 3' | -51.7 | NC_002687.1 | + | 103401 | 0.66 | 0.999015 |
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Target: 5'- gGCUGCAGC-CUUGGCGgccUUGGCug-CAg -3' miRNA: 3'- -CGGCGUUGcGAACCGU---GACUGuaaGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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