miRNA: 3'- -AGGCUAa--CAGGAACCUgcaAGGAAuu -5'
miRNA: 3'- aGGCUAa----CAGGaACCUGCAAGgAAUU- -5'
miRNA: 3'- -AGGcUaaCAGGAACCUGCAa----GGAauu -5'
miRNA: 3'- aGGCUAAcaGGAACCUGCAaGGAAUU- -5'
miRNA: 3'- -AGGCUAACAGGAACCUGCAAGGAAUU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10294 | 3' | -48.9 | NC_002687.1 | + | 304330 | 0.66 | 0.999966 |
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Target: 5'- gUCCGAUggaGUCUUUGGAaa-UCCUUc- -3' miRNA: 3'- -AGGCUAa--CAGGAACCUgcaAGGAAuu -5' |
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| 10294 | 3' | -48.9 | NC_002687.1 | + | 150815 | 0.67 | 0.99979 |
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Target: 5'- aCUGAUgaugaGUCC-UGGACGUUUgUUGAg -3' miRNA: 3'- aGGCUAa----CAGGaACCUGCAAGgAAUU- -5' |
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| 10294 | 3' | -48.9 | NC_002687.1 | + | 122334 | 0.68 | 0.999367 |
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Target: 5'- uUCUuGggGUCCUUGGACGUgcacgCCUg-- -3' miRNA: 3'- -AGGcUaaCAGGAACCUGCAa----GGAauu -5' |
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| 10294 | 3' | -48.9 | NC_002687.1 | + | 22325 | 0.75 | 0.946355 |
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Target: 5'- aCCGAUUcgUCUUGGACGUcCCUUAAa -3' miRNA: 3'- aGGCUAAcaGGAACCUGCAaGGAAUU- -5' |
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| 10294 | 3' | -48.9 | NC_002687.1 | + | 22722 | 1.08 | 0.032729 |
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Target: 5'- aUCCGAUUGUCCUUGGACGUUCCUUAAa -3' miRNA: 3'- -AGGCUAACAGGAACCUGCAAGGAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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