Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10294 | 5' | -47 | NC_002687.1 | + | 150365 | 0.71 | 0.999264 |
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Target: 5'- -----cGGGACGUUCAAGGCuAAUCc -3' miRNA: 3'- aaaauuCCCUGCAGGUUCUGcUUAGc -5' |
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| 10294 | 5' | -47 | NC_002687.1 | + | 310015 | 0.71 | 0.998906 |
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Target: 5'- -cUUGGGGGuccCGUcCCGAGACGAAgucCGg -3' miRNA: 3'- aaAAUUCCCu--GCA-GGUUCUGCUUa--GC- -5' |
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| 10294 | 5' | -47 | NC_002687.1 | + | 22358 | 0.73 | 0.996286 |
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Target: 5'- cUUUUAAGGaACGUCCAAGGacAAUCGg -3' miRNA: 3'- -AAAAUUCCcUGCAGGUUCUgcUUAGC- -5' |
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| 10294 | 5' | -47 | NC_002687.1 | + | 99768 | 0.74 | 0.994481 |
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Target: 5'- ---aGAGGGguguuucggcgcaucGCGUCCAAGACGcGUCu -3' miRNA: 3'- aaaaUUCCC---------------UGCAGGUUCUGCuUAGc -5' |
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| 10294 | 5' | -47 | NC_002687.1 | + | 167998 | 0.78 | 0.950553 |
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Target: 5'- --gUGGGGGugGUgaAGGACGAGUCGc -3' miRNA: 3'- aaaAUUCCCugCAggUUCUGCUUAGC- -5' |
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| 10294 | 5' | -47 | NC_002687.1 | + | 22688 | 1.08 | 0.048918 |
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Target: 5'- cUUUUAAGGGACGUCCAAGACGAAUCGg -3' miRNA: 3'- -AAAAUUCCCUGCAGGUUCUGCUUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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