Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10296 | 3' | -53.4 | NC_002687.1 | + | 5670 | 0.66 | 0.996858 |
Target: 5'- ---cCGGUCGUCcuCGACcagucgagcgAGGCGCUg -3' miRNA: 3'- cacaGCCAGCAGuuGCUG----------UCUGCGAa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 24363 | 0.66 | 0.995746 |
Target: 5'- aGUGacaUCGGUCGUCAcaAgGACGGcgaGCUUg -3' miRNA: 3'- -CAC---AGCCAGCAGU--UgCUGUCug-CGAA- -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 117830 | 0.66 | 0.99508 |
Target: 5'- -cGUCGuccUCGuUCGACG-CAGGCGCUg -3' miRNA: 3'- caCAGCc--AGC-AGUUGCuGUCUGCGAa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 117860 | 0.66 | 0.99508 |
Target: 5'- -cGUCGuccUCGuUCGACG-CAGGCGCUg -3' miRNA: 3'- caCAGCc--AGC-AGUUGCuGUCUGCGAa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 60665 | 0.67 | 0.993493 |
Target: 5'- -gGUUGGUUGUCAAcaagcaguuCGGCAGACaGCc- -3' miRNA: 3'- caCAGCCAGCAGUU---------GCUGUCUG-CGaa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 199620 | 0.67 | 0.991522 |
Target: 5'- --aUCGGU-GUCAAccuCGACGGGCGCa- -3' miRNA: 3'- cacAGCCAgCAGUU---GCUGUCUGCGaa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 135328 | 0.68 | 0.986182 |
Target: 5'- cGUGgccgCGGUCGagcaCGACGaacguGCAGACGCa- -3' miRNA: 3'- -CACa---GCCAGCa---GUUGC-----UGUCUGCGaa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 204980 | 0.69 | 0.974799 |
Target: 5'- cGUGcgCGGUacagaagauuucacgCcGUCGGCGAUAGACGCUg -3' miRNA: 3'- -CACa-GCCA---------------G-CAGUUGCUGUCUGCGAa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 237237 | 0.74 | 0.819049 |
Target: 5'- --uUCGGUCGUCAaugACGGCGGugGUg- -3' miRNA: 3'- cacAGCCAGCAGU---UGCUGUCugCGaa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 122620 | 0.75 | 0.766323 |
Target: 5'- --cUUGG-CGUCGAUGACAGACGCUc -3' miRNA: 3'- cacAGCCaGCAGUUGCUGUCUGCGAa -5' |
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10296 | 3' | -53.4 | NC_002687.1 | + | 25975 | 1.05 | 0.020734 |
Target: 5'- gGUGUCGGUCGUCAACGACAGACGCUUc -3' miRNA: 3'- -CACAGCCAGCAGUUGCUGUCUGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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