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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10296 | 5' | -52 | NC_002687.1 | + | 39559 | 0.69 | 0.993363 |
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Target: 5'- uUCuguCGcCCAUUugacaaucGaCGUCCGCUCCCCu -3' miRNA: 3'- -GGuuuGCaGGUAA--------C-GCAGGUGAGGGG- -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 38462 | 0.66 | 0.999546 |
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Target: 5'- --cGGCaUuuGUUGCGcCCGCUCCCa -3' miRNA: 3'- gguUUGcAggUAACGCaGGUGAGGGg -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 37551 | 0.69 | 0.994207 |
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Target: 5'- aCCAAguuuACGUagaAUUGUGUCUGCUCUUCg -3' miRNA: 3'- -GGUU----UGCAgg-UAACGCAGGUGAGGGG- -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 34220 | 0.67 | 0.998307 |
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Target: 5'- -gAAGCuUCCAUggGUGUCCuuCUCCCg -3' miRNA: 3'- ggUUUGcAGGUAa-CGCAGGu-GAGGGg -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 31774 | 0.68 | 0.996764 |
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Target: 5'- gCCGAAauUCCAUUGCuGUCUACaUCCa- -3' miRNA: 3'- -GGUUUgcAGGUAACG-CAGGUG-AGGgg -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 27099 | 0.67 | 0.997996 |
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Target: 5'- gCCAGGCaGUCCGUagugauUGuUGUCCACaacaCCCUg -3' miRNA: 3'- -GGUUUG-CAGGUA------AC-GCAGGUGa---GGGG- -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 25940 | 1.15 | 0.007879 |
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Target: 5'- uCCAAACGUCCAUUGCGUCCACUCCCCa -3' miRNA: 3'- -GGUUUGCAGGUAACGCAGGUGAGGGG- -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 22035 | 0.73 | 0.946915 |
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Target: 5'- uCCuucAugGUCU----CGUCCGCUCCCCa -3' miRNA: 3'- -GGu--UugCAGGuaacGCAGGUGAGGGG- -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 11063 | 0.69 | 0.992422 |
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Target: 5'- aCCAcacacAACGcccgCCAUgcugagaGCGUCCGCUUCCg -3' miRNA: 3'- -GGU-----UUGCa---GGUAa------CGCAGGUGAGGGg -5' |
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| 10296 | 5' | -52 | NC_002687.1 | + | 10753 | 0.71 | 0.978444 |
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Target: 5'- aCCAcggucGCGUCCGcUGCcuucUCCAC-CCCCg -3' miRNA: 3'- -GGUu----UGCAGGUaACGc---AGGUGaGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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