Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10298 | 3' | -50.6 | NC_002687.1 | + | 168963 | 0.66 | 0.999863 |
Target: 5'- -gGAAAUgGUGgagGCCGCUCa-GGAGAu -3' miRNA: 3'- gaCUUUGgCACa--CGGUGAGcaCUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 279642 | 0.66 | 0.99986 |
Target: 5'- uCUGGugGGCCGgugGUGCCggcaccaccugcuGCUCGUGGu-- -3' miRNA: 3'- -GACU--UUGGCa--CACGG-------------UGAGCACUucu -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 37094 | 0.66 | 0.999724 |
Target: 5'- uUGAAAUgGUGguUGCCAUg-GUGAAGGu -3' miRNA: 3'- gACUUUGgCAC--ACGGUGagCACUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 38399 | 0.66 | 0.999724 |
Target: 5'- uCUGAAaacgGCCaGUGaaaauUGCauuGCUCGUGAAGGa -3' miRNA: 3'- -GACUU----UGG-CAC-----ACGg--UGAGCACUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 263142 | 0.67 | 0.999042 |
Target: 5'- gUGAAugCGUGUGCCGUUCGa----- -3' miRNA: 3'- gACUUugGCACACGGUGAGCacuucu -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 279410 | 0.68 | 0.998612 |
Target: 5'- uUGAGGCCGcugGUGUCAC-CaGUGAAGc -3' miRNA: 3'- gACUUUGGCa--CACGGUGaG-CACUUCu -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 20721 | 0.69 | 0.99562 |
Target: 5'- -cGAAgGCCacGUGCUugUCGUGGAGGa -3' miRNA: 3'- gaCUU-UGGcaCACGGugAGCACUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 7599 | 0.69 | 0.994147 |
Target: 5'- -aGAAGCCG-GUGCCAgUgGUGAc-- -3' miRNA: 3'- gaCUUUGGCaCACGGUgAgCACUucu -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 259690 | 0.72 | 0.97526 |
Target: 5'- uUGAu-CCGUGUGCCACUuccuagCGcGGAGAa -3' miRNA: 3'- gACUuuGGCACACGGUGA------GCaCUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 43970 | 0.73 | 0.962667 |
Target: 5'- -gGAAACCaaucuuaaauacguGUGUGCCAa-CGUGAAGAa -3' miRNA: 3'- gaCUUUGG--------------CACACGGUgaGCACUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 13184 | 0.74 | 0.930195 |
Target: 5'- -aGAGGgCGUGUGCCACUggagcgacacaGUGAGGAa -3' miRNA: 3'- gaCUUUgGCACACGGUGAg----------CACUUCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 303578 | 0.79 | 0.766876 |
Target: 5'- uUGucGGCCGUGUGCCugUCGUGccuuGGAu -3' miRNA: 3'- gACu-UUGGCACACGGugAGCACu---UCU- -5' |
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10298 | 3' | -50.6 | NC_002687.1 | + | 40341 | 1.09 | 0.02 |
Target: 5'- gCUGAAACCGUGUGCCACUCGUGAAGAc -3' miRNA: 3'- -GACUUUGGCACACGGUGAGCACUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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