Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10298 | 5' | -54.5 | NC_002687.1 | + | 64409 | 0.66 | 0.993082 |
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Target: 5'- aGUUUUUCCCaC-GUUGUCCACCGa--- -3' miRNA: 3'- -CAAGAAGGG-GaCAGCAGGUGGUguca -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 268163 | 0.66 | 0.992801 |
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Target: 5'- -aUCUUUCaCCcGUCGUucgaagccguggugCCACCACAGc -3' miRNA: 3'- caAGAAGG-GGaCAGCA--------------GGUGGUGUCa -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 170042 | 0.66 | 0.988529 |
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Target: 5'- --cCUUUUCCgacaUCGUCCACCACAa- -3' miRNA: 3'- caaGAAGGGGac--AGCAGGUGGUGUca -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 317885 | 0.67 | 0.985515 |
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Target: 5'- uUUUUUCUCUUGUUGUuaUCGCCAUAGg -3' miRNA: 3'- cAAGAAGGGGACAGCA--GGUGGUGUCa -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 202664 | 0.67 | 0.977719 |
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Target: 5'- uGUUCUUCCCCccg-GUCCGCgaCGCGGUu -3' miRNA: 3'- -CAAGAAGGGGacagCAGGUG--GUGUCA- -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 203101 | 0.67 | 0.977719 |
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Target: 5'- uGUUCUUCCCCccg-GUCCGCgaCGCGGUu -3' miRNA: 3'- -CAAGAAGGGGacagCAGGUG--GUGUCA- -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 18408 | 0.68 | 0.969252 |
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Target: 5'- -gUCguugUCCCCuuuggugaaaucgcUGUCG-CUACCACAGUc -3' miRNA: 3'- caAGa---AGGGG--------------ACAGCaGGUGGUGUCA- -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 209570 | 0.68 | 0.967195 |
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Target: 5'- --aUUUCCCCUGUCGUCUcuauguACaCACAa- -3' miRNA: 3'- caaGAAGGGGACAGCAGG------UG-GUGUca -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 211173 | 0.69 | 0.955076 |
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Target: 5'- -gUCUUCCUCggggGUCGUCaCcgugagcgaugcaugGCCGCAGUg -3' miRNA: 3'- caAGAAGGGGa---CAGCAG-G---------------UGGUGUCA- -5' |
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| 10298 | 5' | -54.5 | NC_002687.1 | + | 40304 | 1.06 | 0.012515 |
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Target: 5'- gGUUCUUCCCCUGUCGUCCACCACAGUu -3' miRNA: 3'- -CAAGAAGGGGACAGCAGGUGGUGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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