Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10299 | 3' | -53.4 | NC_002687.1 | + | 203701 | 0.66 | 0.996076 |
Target: 5'- aGAagaGUCgaacggCAGGAGAaGAgcuucuucGUUGGCCAc -3' miRNA: 3'- aCUg--CAGa-----GUCCUCUaCU--------CAACCGGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 325454 | 0.66 | 0.994746 |
Target: 5'- uUGugGUCg-AGGAGGggaccacagGAGUgucGGCCAc -3' miRNA: 3'- -ACugCAGagUCCUCUa--------CUCAa--CCGGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 171726 | 0.66 | 0.993956 |
Target: 5'- aUGugGUUaCGGuGAcGAUGAGUUGGaCCc -3' miRNA: 3'- -ACugCAGaGUC-CU-CUACUCAACC-GGu -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 142522 | 0.66 | 0.993701 |
Target: 5'- gGACGUCUC-GGAGAUGuaccucagccuGUcgucgucguccucgUGGCCu -3' miRNA: 3'- aCUGCAGAGuCCUCUACu----------CA--------------ACCGGu -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 130477 | 0.67 | 0.989791 |
Target: 5'- aUGAUGccuuUCUCGuucGGUGGGUUGGCCAu -3' miRNA: 3'- -ACUGC----AGAGUccuCUACUCAACCGGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 320730 | 0.67 | 0.989791 |
Target: 5'- cGuCGUCaUCAGGAGAcucGAGcagGGCCu -3' miRNA: 3'- aCuGCAG-AGUCCUCUa--CUCaa-CCGGu -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 2959 | 0.67 | 0.989791 |
Target: 5'- cGAUGUCg-AGaGGAUGGGUUGGaCCGa -3' miRNA: 3'- aCUGCAGagUCcUCUACUCAACC-GGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 245185 | 0.67 | 0.989791 |
Target: 5'- cGuCGUCaUCAGGAGAcucGAGcagGGCCu -3' miRNA: 3'- aCuGCAG-AGUCCUCUa--CUCaa-CCGGu -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 267878 | 0.69 | 0.972497 |
Target: 5'- aGGuCGgugCUCAGGAGGUGc-UUGGCCc -3' miRNA: 3'- aCU-GCa--GAGUCCUCUACucAACCGGu -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 270647 | 0.69 | 0.963601 |
Target: 5'- uUGAUGUgcaagucggugCUCAGGAGGUGc-UUGGCCc -3' miRNA: 3'- -ACUGCA-----------GAGUCCUCUACucAACCGGu -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 280528 | 0.73 | 0.854609 |
Target: 5'- gGAUGUCUCGGGA-AUG-GUUGGCgCAa -3' miRNA: 3'- aCUGCAGAGUCCUcUACuCAACCG-GU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 309035 | 0.73 | 0.836666 |
Target: 5'- cGACGUC-CAGGGGAucagagcuuugaagUGAGccUGGCCGu -3' miRNA: 3'- aCUGCAGaGUCCUCU--------------ACUCa-ACCGGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 247348 | 0.98 | 0.052724 |
Target: 5'- cUGACcUCUCAGGAGAUGAGUUGGCCAc -3' miRNA: 3'- -ACUGcAGAGUCCUCUACUCAACCGGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 40713 | 1.08 | 0.011515 |
Target: 5'- cUGACGUCUCAGGAGAUGAGUUGGCCAc -3' miRNA: 3'- -ACUGCAGAGUCCUCUACUCAACCGGU- -5' |
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10299 | 3' | -53.4 | NC_002687.1 | + | 318567 | 1.08 | 0.011515 |
Target: 5'- cUGACGUCUCAGGAGAUGAGUUGGCCAc -3' miRNA: 3'- -ACUGCAGAGUCCUCUACUCAACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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