Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
103 | 5' | -57.8 | AC_000006.1 | + | 19743 | 0.66 | 0.450805 |
Target: 5'- -cUCGCGCaacugGGCCGCUUUUCGc-GGCu -3' miRNA: 3'- gaAGCGCG-----CUGGCGAAGGGCauCCGu -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 15476 | 0.66 | 0.418504 |
Target: 5'- --cCGCGCG-CCGCgccgccacugcacccCCCGcAGGCAg -3' miRNA: 3'- gaaGCGCGCuGGCGaa-------------GGGCaUCCGU- -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 12369 | 0.67 | 0.412783 |
Target: 5'- --cUGCGCgGGCUGUggggcgugcaggcgCCCGUGGGCGa -3' miRNA: 3'- gaaGCGCG-CUGGCGaa------------GGGCAUCCGU- -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 10864 | 0.67 | 0.375019 |
Target: 5'- --aUGCGCGACUGCcggUUUCGggcGGGCAg -3' miRNA: 3'- gaaGCGCGCUGGCGa--AGGGCa--UCCGU- -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 15607 | 0.67 | 0.36617 |
Target: 5'- --aCGgGCGugCGCgugCCCGUGcGCAc -3' miRNA: 3'- gaaGCgCGCugGCGaa-GGGCAUcCGU- -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 34927 | 0.68 | 0.357465 |
Target: 5'- --cCGCGCGACCGUUagCCGUGcGUc -3' miRNA: 3'- gaaGCGCGCUGGCGAagGGCAUcCGu -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 174 | 0.68 | 0.357465 |
Target: 5'- --cCGCGCGACCGUUagCCGUGcGUc -3' miRNA: 3'- gaaGCGCGCUGGCGAagGGCAUcCGu -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 17425 | 0.68 | 0.340496 |
Target: 5'- -cUgGcCGCGGCCGCUUCUCGcccuucGGCGg -3' miRNA: 3'- gaAgC-GCGCUGGCGAAGGGCau----CCGU- -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 15517 | 0.69 | 0.316151 |
Target: 5'- --gCGCGCGGCuCGCaucauggCCCGgcGGCc -3' miRNA: 3'- gaaGCGCGCUG-GCGaa-----GGGCauCCGu -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 8314 | 0.72 | 0.186998 |
Target: 5'- -gUCGCG-GACgCGC-UCCCGgcGGCAg -3' miRNA: 3'- gaAGCGCgCUG-GCGaAGGGCauCCGU- -5' |
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103 | 5' | -57.8 | AC_000006.1 | + | 16069 | 1.08 | 0.000363 |
Target: 5'- uCUUCGCGCGACCGCUUCCCGUAGGCAa -3' miRNA: 3'- -GAAGCGCGCUGGCGAAGGGCAUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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