Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10301 | 5' | -52.9 | NC_002687.1 | + | 181566 | 0.67 | 0.991494 |
Target: 5'- -cGGGCGaaaCGUGCUUCuaucgcgcuauGUGCuCGGUGGa -3' miRNA: 3'- uuCUCGCa--GCAUGAAG-----------UACG-GCCACU- -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 166545 | 0.67 | 0.99033 |
Target: 5'- aGAGGGUGUCGUGCgcgaaGCgGGUGu -3' miRNA: 3'- -UUCUCGCAGCAUGaaguaCGgCCACu -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 159847 | 0.68 | 0.989046 |
Target: 5'- uGGAGCuccaGUCGUaagcgacaggagACUUCAUGUCGGUc- -3' miRNA: 3'- uUCUCG----CAGCA------------UGAAGUACGGCCAcu -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 226932 | 0.7 | 0.964722 |
Target: 5'- -cGGGCGUCGccccgucgGCUccgUCGgcUGCCGGUGGu -3' miRNA: 3'- uuCUCGCAGCa-------UGA---AGU--ACGGCCACU- -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 50220 | 0.72 | 0.89806 |
Target: 5'- -cGuGCGUCGUGCU--GUGCUGGUGc -3' miRNA: 3'- uuCuCGCAGCAUGAagUACGGCCACu -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 51107 | 0.72 | 0.89806 |
Target: 5'- -cGuGCGUCGUGCU--GUGCUGGUGc -3' miRNA: 3'- uuCuCGCAGCAUGAagUACGGCCACu -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 52092 | 0.72 | 0.89806 |
Target: 5'- -cGuGCGUCGUGCU--GUGCUGGUGc -3' miRNA: 3'- uuCuCGCAGCAUGAagUACGGCCACu -5' |
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10301 | 5' | -52.9 | NC_002687.1 | + | 43214 | 1.07 | 0.015026 |
Target: 5'- cAAGAGCGUCGUACUUCAUGCCGGUGAa -3' miRNA: 3'- -UUCUCGCAGCAUGAAGUACGGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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