Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10302 | 3' | -54.8 | NC_002687.1 | + | 24131 | 0.66 | 0.991879 |
Target: 5'- -aGCgGGGAGgUGCCuUUCGGGCGCa-- -3' miRNA: 3'- agCG-UCCUCaACGG-AGGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 48151 | 0.66 | 0.986773 |
Target: 5'- aUGCcgacuGGGGGgacaGCCUCCGGugGCg-- -3' miRNA: 3'- aGCG-----UCCUCaa--CGGAGGCUugCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 12950 | 0.66 | 0.985176 |
Target: 5'- gUCGCAGGAGcgcaGCCgaUCacaGAGCGCcGGc -3' miRNA: 3'- -AGCGUCCUCaa--CGG--AGg--CUUGCGaUC- -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 304225 | 0.67 | 0.983435 |
Target: 5'- cUGCAGcuacGGUUG-CUCCGGGgGCUAGg -3' miRNA: 3'- aGCGUCc---UCAACgGAGGCUUgCGAUC- -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 90011 | 0.67 | 0.974901 |
Target: 5'- gUCGCGGGAGcaggUGCCguaagaUCGGGCaCUGGa -3' miRNA: 3'- -AGCGUCCUCa---ACGGa-----GGCUUGcGAUC- -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 279362 | 0.68 | 0.97234 |
Target: 5'- aCGCuGGAG-UGCCgUCCGGuuccacugcCGCUGGu -3' miRNA: 3'- aGCGuCCUCaACGG-AGGCUu--------GCGAUC- -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 14309 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 14641 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 14906 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 15168 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 15499 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 15765 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 16026 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 16357 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 16622 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 16953 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 14048 | 0.68 | 0.956682 |
Target: 5'- gUGUAGG--UUGCCUgCCGAGCGCa-- -3' miRNA: 3'- aGCGUCCucAACGGA-GGCUUGCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 259941 | 0.7 | 0.931042 |
Target: 5'- -gGCAGGAGUgccaGCCUCCauuauuuuGAaaACGCUGa -3' miRNA: 3'- agCGUCCUCAa---CGGAGG--------CU--UGCGAUc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 309200 | 0.74 | 0.736901 |
Target: 5'- -aGCGGGAG--GCCUCCGGugGCa-- -3' miRNA: 3'- agCGUCCUCaaCGGAGGCUugCGauc -5' |
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10302 | 3' | -54.8 | NC_002687.1 | + | 46189 | 0.97 | 0.047468 |
Target: 5'- cUCGCAGGAGUUGCCU-CGAACGCUAGg -3' miRNA: 3'- -AGCGUCCUCAACGGAgGCUUGCGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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