Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10302 | 5' | -57.2 | NC_002687.1 | + | 277828 | 0.66 | 0.976843 |
Target: 5'- gCCGGG-GAUUGaUGACUUGCAgaGa -3' miRNA: 3'- gGGCCCuCUAGCcACUGGACGUgaCg -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 115540 | 0.66 | 0.974499 |
Target: 5'- cCCCGGuGGcAUCGGagcucUGGCgUGCGUUGCg -3' miRNA: 3'- -GGGCCcUC-UAGCC-----ACUGgACGUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 135478 | 0.66 | 0.974011 |
Target: 5'- aCCCGu--GAUCGGUGACCcauuuuucaccgGCAUUGa -3' miRNA: 3'- -GGGCccuCUAGCCACUGGa-----------CGUGACg -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 209632 | 0.66 | 0.963393 |
Target: 5'- aCCCGGGuGGUCcccaGGUGcuCgUGCAgaGCc -3' miRNA: 3'- -GGGCCCuCUAG----CCACu-GgACGUgaCG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 172199 | 0.67 | 0.960158 |
Target: 5'- aCCUGGGccAGAUCcuggaGUGGCCgcuucUGCGuCUGCg -3' miRNA: 3'- -GGGCCC--UCUAGc----CACUGG-----ACGU-GACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 133662 | 0.67 | 0.960158 |
Target: 5'- aCCCGGGAucuucCGGUGugCUu--CUGCa -3' miRNA: 3'- -GGGCCCUcua--GCCACugGAcguGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 279329 | 0.67 | 0.949288 |
Target: 5'- -gUGGGAGcAUCGcUGGgucugccccccCCUGCGCUGCc -3' miRNA: 3'- ggGCCCUC-UAGCcACU-----------GGACGUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 97494 | 0.67 | 0.945266 |
Target: 5'- gUCGGGuuaGGGUCuugugGGUGA-CUGUACUGCu -3' miRNA: 3'- gGGCCC---UCUAG-----CCACUgGACGUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 240107 | 0.68 | 0.94104 |
Target: 5'- gCUCaGGaAGAUCGGUGAuCCgugcaGCGCaUGCa -3' miRNA: 3'- -GGGcCC-UCUAGCCACU-GGa----CGUG-ACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 156813 | 0.68 | 0.936608 |
Target: 5'- uCCCa-GAGGuauUCGGUGACUgaccccgGCAUUGCg -3' miRNA: 3'- -GGGccCUCU---AGCCACUGGa------CGUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 79221 | 0.69 | 0.893711 |
Target: 5'- gCUCGGaAGAUUGGaaaccgacgUGGCC-GCGCUGCg -3' miRNA: 3'- -GGGCCcUCUAGCC---------ACUGGaCGUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 137315 | 0.69 | 0.887433 |
Target: 5'- uCCCGGGAuuUUGaaGUGAagaCUGCugUGCc -3' miRNA: 3'- -GGGCCCUcuAGC--CACUg--GACGugACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 309382 | 0.69 | 0.880959 |
Target: 5'- cCCCuGGAcGUCGGUGAguCCUG-ACUGUg -3' miRNA: 3'- -GGGcCCUcUAGCCACU--GGACgUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 332500 | 0.69 | 0.880301 |
Target: 5'- uCCCuGGGGA-CGGUGAUCcacccuuUGCgGCUGCa -3' miRNA: 3'- -GGGcCCUCUaGCCACUGG-------ACG-UGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 135311 | 0.7 | 0.867439 |
Target: 5'- gCCGGGAGcUCGGUGuCCUGgAUc-- -3' miRNA: 3'- gGGCCCUCuAGCCACuGGACgUGacg -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 37231 | 0.71 | 0.822631 |
Target: 5'- --gGGGAGAUUGGUGACUauuuauCACUGUg -3' miRNA: 3'- gggCCCUCUAGCCACUGGac----GUGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 223590 | 0.72 | 0.78455 |
Target: 5'- uCCCGGGGGAggggggUGGUGGCC-GCAacacaccacguucuuCUGUg -3' miRNA: 3'- -GGGCCCUCUa-----GCCACUGGaCGU---------------GACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 18869 | 0.73 | 0.699689 |
Target: 5'- cCuuGGGGGcgCGGUGGCCUcCuucuGCUGCu -3' miRNA: 3'- -GggCCCUCuaGCCACUGGAcG----UGACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 43617 | 0.76 | 0.548143 |
Target: 5'- gCgGGGAGcGUCGGUcuaGACCUGCAgUGUa -3' miRNA: 3'- gGgCCCUC-UAGCCA---CUGGACGUgACG- -5' |
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10302 | 5' | -57.2 | NC_002687.1 | + | 46156 | 1.13 | 0.003084 |
Target: 5'- uCCCGGGAGAUCGGUGACCUGCACUGCa -3' miRNA: 3'- -GGGCCCUCUAGCCACUGGACGUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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