Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10305 | 5' | -59.9 | NC_002687.1 | + | 252594 | 0.72 | 0.617899 |
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Target: 5'- aGAUcAUGCGCCCGgaugGCGuCCCGGgACCGAg -3' miRNA: 3'- -UUGuUGCGCGGGC----UGU-GGGCC-UGGCU- -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 257392 | 0.79 | 0.272652 |
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Target: 5'- cGCGAUGUGCCCGACgcGCUCGGGCCa- -3' miRNA: 3'- uUGUUGCGCGGGCUG--UGGGCCUGGcu -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 298975 | 0.68 | 0.839588 |
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Target: 5'- gAGCAGCGCugcGCCCG-CcUCCGGAgcaCCGAg -3' miRNA: 3'- -UUGUUGCG---CGGGCuGuGGGCCU---GGCU- -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 303852 | 0.66 | 0.894399 |
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Target: 5'- cAGCAGCGgGCCUaGCcCCCGGAgCa- -3' miRNA: 3'- -UUGUUGCgCGGGcUGuGGGCCUgGcu -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 308789 | 0.7 | 0.739213 |
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Target: 5'- aGGgGGCGCGCCCGcccCGCCgGGgucGCCGGg -3' miRNA: 3'- -UUgUUGCGCGGGCu--GUGGgCC---UGGCU- -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 321975 | 0.73 | 0.570862 |
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Target: 5'- cACAGCGCGCCUugaGGCACCU-GAUCGAa -3' miRNA: 3'- uUGUUGCGCGGG---CUGUGGGcCUGGCU- -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 323499 | 0.67 | 0.877208 |
|
Target: 5'- gGACGACGCGCCggcaucucucuuuCGGCAaggugaggcgucccuCCUGGugUGAa -3' miRNA: 3'- -UUGUUGCGCGG-------------GCUGU---------------GGGCCugGCU- -5' |
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| 10305 | 5' | -59.9 | NC_002687.1 | + | 333321 | 0.67 | 0.881799 |
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Target: 5'- cAACAuACGCGCUCGACACCaagaauauggcaCGuGGCCu- -3' miRNA: 3'- -UUGU-UGCGCGGGCUGUGG------------GC-CUGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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