Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10308 | 3' | -47.1 | NC_002687.1 | + | 294952 | 0.7 | 0.999515 |
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Target: 5'- ---cUCGAgCAAcGCCUGCcUGUaGGCg -3' miRNA: 3'- uuuaAGCUaGUUuCGGACGaACA-CCG- -5' |
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| 10308 | 3' | -47.1 | NC_002687.1 | + | 83300 | 0.7 | 0.999258 |
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Target: 5'- ---aUCGAUCGAgacauGGCCgggggGUUUGcUGGCa -3' miRNA: 3'- uuuaAGCUAGUU-----UCGGa----CGAAC-ACCG- -5' |
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| 10308 | 3' | -47.1 | NC_002687.1 | + | 293703 | 0.71 | 0.998656 |
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Target: 5'- ---gUCGAUCAAAaCUUGCUUGagcaggGGCg -3' miRNA: 3'- uuuaAGCUAGUUUcGGACGAACa-----CCG- -5' |
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| 10308 | 3' | -47.1 | NC_002687.1 | + | 331219 | 0.71 | 0.99838 |
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Target: 5'- ----gCGAgc-GGGCCUGCU-GUGGCg -3' miRNA: 3'- uuuaaGCUaguUUCGGACGAaCACCG- -5' |
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| 10308 | 3' | -47.1 | NC_002687.1 | + | 125976 | 0.77 | 0.938961 |
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Target: 5'- cGGAUguaGUCAAAGCCUGCUUacaGUGGCc -3' miRNA: 3'- -UUUAagcUAGUUUCGGACGAA---CACCG- -5' |
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| 10308 | 3' | -47.1 | NC_002687.1 | + | 64975 | 1.12 | 0.025713 |
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Target: 5'- aAAAUUCGAUCAAAGCCUGCUUGUGGCa -3' miRNA: 3'- -UUUAAGCUAGUUUCGGACGAACACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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