Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10308 | 5' | -60.1 | NC_002687.1 | + | 97320 | 0.68 | 0.781846 |
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Target: 5'- aGGGCuCCGGuGCagGCCUCGACaucgaCCGAc -3' miRNA: 3'- -CUCG-GGUCuCGaaCGGAGCUGg----GGUU- -5' |
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| 10308 | 5' | -60.1 | NC_002687.1 | + | 126070 | 0.68 | 0.755114 |
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Target: 5'- uGAGCCCGGcaccaaGGCUcGCCUUGuuUCCCGAg -3' miRNA: 3'- -CUCGGGUC------UCGAaCGGAGCu-GGGGUU- -5' |
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| 10308 | 5' | -60.1 | NC_002687.1 | + | 103817 | 0.68 | 0.745999 |
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Target: 5'- aGGCCCGGu-CUUGCCaacCGAUCCCAGc -3' miRNA: 3'- cUCGGGUCucGAACGGa--GCUGGGGUU- -5' |
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| 10308 | 5' | -60.1 | NC_002687.1 | + | 32323 | 0.71 | 0.612505 |
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Target: 5'- aAGUCCAGAGCgUGCUgcgCGAcuaCCCCAAu -3' miRNA: 3'- cUCGGGUCUCGaACGGa--GCU---GGGGUU- -5' |
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| 10308 | 5' | -60.1 | NC_002687.1 | + | 44965 | 0.71 | 0.602844 |
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Target: 5'- aAGCCgAGAGCUuucUGCCU--ACCCCAGg -3' miRNA: 3'- cUCGGgUCUCGA---ACGGAgcUGGGGUU- -5' |
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| 10308 | 5' | -60.1 | NC_002687.1 | + | 64904 | 0.71 | 0.574002 |
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Target: 5'- cGAGCCCAGGGCUUGagUgGugUCCGg -3' miRNA: 3'- -CUCGGGUCUCGAACggAgCugGGGUu -5' |
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| 10308 | 5' | -60.1 | NC_002687.1 | + | 64937 | 1.06 | 0.003572 |
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Target: 5'- cGAGCCCAGAGCUUGCCUCGACCCCAAa -3' miRNA: 3'- -CUCGGGUCUCGAACGGAGCUGGGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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