Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10311 | 3' | -50.8 | NC_002687.1 | + | 238787 | 0.69 | 0.99221 |
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Target: 5'- -cACCAUggUGUGGCGUUGUUGUgGCg- -3' miRNA: 3'- aaUGGUA--GCGCUGUAACAACAgCGgc -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 84713 | 0.69 | 0.99221 |
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Target: 5'- --gUCGUCGCGACGUUGacgGUUGCgGc -3' miRNA: 3'- aauGGUAGCGCUGUAACaa-CAGCGgC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 59412 | 0.72 | 0.972266 |
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Target: 5'- gUGCCGUCGCucGACAUcGUcgUGUCGgCGg -3' miRNA: 3'- aAUGGUAGCG--CUGUAaCA--ACAGCgGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 60375 | 0.72 | 0.971991 |
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Target: 5'- gUACCAaucccgaUCGCGuACAUUGUUGUCcUCGa -3' miRNA: 3'- aAUGGU-------AGCGC-UGUAACAACAGcGGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 26168 | 0.76 | 0.850706 |
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Target: 5'- -cGCCGUUGCGcCGgagggUGUUGUCGUCGa -3' miRNA: 3'- aaUGGUAGCGCuGUa----ACAACAGCGGC- -5' |
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| 10311 | 3' | -50.8 | NC_002687.1 | + | 69886 | 1.08 | 0.022848 |
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Target: 5'- uUUACCAUCGCGACAUUGUUGUCGCCGa -3' miRNA: 3'- -AAUGGUAGCGCUGUAACAACAGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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