Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10313 | 5' | -58.7 | NC_002687.1 | + | 282061 | 0.68 | 0.867943 |
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Target: 5'- cGGCaggGGCCGUAGUggcagcaGCGgcgGCUCGGg -3' miRNA: 3'- -CCG---CCGGCGUCAagag---CGCa--CGAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 10334 | 0.69 | 0.846428 |
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Target: 5'- uGGCGGCgGCAGUUgUgGCaGUGUcaucCGAa -3' miRNA: 3'- -CCGCCGgCGUCAAgAgCG-CACGa---GCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 63060 | 0.69 | 0.831238 |
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Target: 5'- uGGUGGCCGCGGUcgaaucagguucUCUUGCucuUGUUCa- -3' miRNA: 3'- -CCGCCGGCGUCA------------AGAGCGc--ACGAGcu -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 99544 | 0.69 | 0.823406 |
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Target: 5'- aGGCGGUcguCGCGGcgCUCGCGcGaUCGAu -3' miRNA: 3'- -CCGCCG---GCGUCaaGAGCGCaCgAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 44138 | 0.71 | 0.746082 |
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Target: 5'- -cCGGCCGCAuccaUCUCGUcauaaugGUGCUCGAu -3' miRNA: 3'- ccGCCGGCGUca--AGAGCG-------CACGAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 298061 | 0.74 | 0.559518 |
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Target: 5'- cGCGGCCGCcGccgCgCGCGUGCUUGGa -3' miRNA: 3'- cCGCCGGCGuCaa-GaGCGCACGAGCU- -5' |
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| 10313 | 5' | -58.7 | NC_002687.1 | + | 78050 | 1.11 | 0.003093 |
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Target: 5'- cGGCGGCCGCAGUUCUCGCGUGCUCGAg -3' miRNA: 3'- -CCGCCGGCGUCAAGAGCGCACGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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