miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10316 5' -48.8 NC_002687.1 + 205840 0.66 0.999963
Target:  5'- gAGCUuggacgUCACCGgugugcaGGCUgagGUGCGUAUAg -3'
miRNA:   3'- gUCGA------AGUGGCa------UUGGa--CACGUAUAU- -5'
10316 5' -48.8 NC_002687.1 + 89454 0.66 0.999951
Target:  5'- cUAGCaugUCACCaaUGACCUGUGuCGUGa- -3'
miRNA:   3'- -GUCGa--AGUGGc-AUUGGACAC-GUAUau -5'
10316 5' -48.8 NC_002687.1 + 216086 0.66 0.999942
Target:  5'- aCAGCUUCGCCG-AAUCUGgauuuuacguggggGCuUAUGg -3'
miRNA:   3'- -GUCGAAGUGGCaUUGGACa-------------CGuAUAU- -5'
10316 5' -48.8 NC_002687.1 + 257618 0.66 0.999916
Target:  5'- gCGGCUg-GCCGagGACCcuuUGUGCAUGUc -3'
miRNA:   3'- -GUCGAagUGGCa-UUGG---ACACGUAUAu -5'
10316 5' -48.8 NC_002687.1 + 170465 0.67 0.999859
Target:  5'- gCAGCUUC-CCGUGAUgUcgcuccUGCAUAUAg -3'
miRNA:   3'- -GUCGAAGuGGCAUUGgAc-----ACGUAUAU- -5'
10316 5' -48.8 NC_002687.1 + 304579 0.67 0.99977
Target:  5'- uCAGCUUCGCC--AACaaaUGuUGCAUGUGu -3'
miRNA:   3'- -GUCGAAGUGGcaUUGg--AC-ACGUAUAU- -5'
10316 5' -48.8 NC_002687.1 + 112211 0.68 0.999436
Target:  5'- uCGGCacaaCGCCugaGUAuCCUGUGCAUGUGa -3'
miRNA:   3'- -GUCGaa--GUGG---CAUuGGACACGUAUAU- -5'
10316 5' -48.8 NC_002687.1 + 50891 0.68 0.999305
Target:  5'- aAGUgagCGCCGUGGCUcaaGUGCAUGUu -3'
miRNA:   3'- gUCGaa-GUGGCAUUGGa--CACGUAUAu -5'
10316 5' -48.8 NC_002687.1 + 243290 0.69 0.998742
Target:  5'- gCGGCgUCGCC-UAGCUUGUGCGaGUAu -3'
miRNA:   3'- -GUCGaAGUGGcAUUGGACACGUaUAU- -5'
10316 5' -48.8 NC_002687.1 + 125760 0.71 0.992477
Target:  5'- uCAGCUUcCACgCGUucCCUGUGCAa--- -3'
miRNA:   3'- -GUCGAA-GUG-GCAuuGGACACGUauau -5'
10316 5' -48.8 NC_002687.1 + 83010 1.07 0.03103
Target:  5'- gCAGCUUCACCGUAACCUGUGCAUAUAa -3'
miRNA:   3'- -GUCGAAGUGGCAUUGGACACGUAUAU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.