Results 21 - 22 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10317 | 3' | -55.7 | NC_002687.1 | + | 175103 | 0.78 | 0.551433 |
Target: 5'- -uCCGGCGGUuccGgcGGUUCCGGCGGUu -3' miRNA: 3'- guGGCUGCCA---CauCCAAGGCUGCCAc -5' |
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10317 | 3' | -55.7 | NC_002687.1 | + | 83751 | 1.08 | 0.009574 |
Target: 5'- aCACCGACGGUGUAGGUUCCGACGGUGu -3' miRNA: 3'- -GUGGCUGCCACAUCCAAGGCUGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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