Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10319 | 5' | -56.7 | NC_002687.1 | + | 295035 | 0.66 | 0.97584 |
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Target: 5'- cGCgGGUGAUGUaCUGUAUucccaCGAGCu-- -3' miRNA: 3'- aCGgCCACUGCA-GACAUG-----GCUCGcac -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 103676 | 0.67 | 0.954908 |
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Target: 5'- uUGCCGGUGGCGaaagCUuu-CUGGGCGa- -3' miRNA: 3'- -ACGGCCACUGCa---GAcauGGCUCGCac -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 239552 | 0.68 | 0.939065 |
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Target: 5'- cGCCGGUGACGaagagauaaagaaagC-GUACCG-GCGUc -3' miRNA: 3'- aCGGCCACUGCa--------------GaCAUGGCuCGCAc -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 199797 | 0.68 | 0.924197 |
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Target: 5'- cGgUGGUGGCGaUUGgcACCGAGCGUa -3' miRNA: 3'- aCgGCCACUGCaGACa-UGGCUCGCAc -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 226791 | 0.68 | 0.924197 |
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Target: 5'- gUGCCGGUGcCGgugCcgGUGCCGguGGCGgUGg -3' miRNA: 3'- -ACGGCCACuGCa--Ga-CAUGGC--UCGC-AC- -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 203353 | 0.69 | 0.889669 |
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Target: 5'- aUGCCGGUGAUGUCgucGUgagucACCGuG-GUGg -3' miRNA: 3'- -ACGGCCACUGCAGa--CA-----UGGCuCgCAC- -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 305288 | 0.7 | 0.862606 |
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Target: 5'- gUGCaUGGUGAUGaacCUGgucgGCCGAGCGUu -3' miRNA: 3'- -ACG-GCCACUGCa--GACa---UGGCUCGCAc -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 205061 | 0.72 | 0.765265 |
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Target: 5'- gGCacauGGUGACGUCcGUGCCcacAGCGUGu -3' miRNA: 3'- aCGg---CCACUGCAGaCAUGGc--UCGCAC- -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 97284 | 0.73 | 0.738079 |
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Target: 5'- uUGCCGGUGGauaUCUGcucgACCGGGCGg- -3' miRNA: 3'- -ACGGCCACUgc-AGACa---UGGCUCGCac -5' |
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| 10319 | 5' | -56.7 | NC_002687.1 | + | 84887 | 1.09 | 0.0062 |
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Target: 5'- aUGCCGGUGACGUCUGUACCGAGCGUGa -3' miRNA: 3'- -ACGGCCACUGCAGACAUGGCUCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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