Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10320 | 3' | -55.3 | NC_002687.1 | + | 209262 | 0.66 | 0.990637 |
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Target: 5'- aUGgGCUCUgcacgAG-CaCCUGGGGACCa -3' miRNA: 3'- gACaUGGGAaa---UCuG-GGACCCCUGGc -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 77103 | 0.66 | 0.986623 |
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Target: 5'- ---gGCCCUcgGGAcacCCUUGGGGAUUGu -3' miRNA: 3'- gacaUGGGAaaUCU---GGGACCCCUGGC- -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 41712 | 0.66 | 0.985026 |
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Target: 5'- gCUGUugCCUaaccaAGACCCggggcuuGGGAUCGa -3' miRNA: 3'- -GACAugGGAaa---UCUGGGac-----CCCUGGC- -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 85655 | 0.66 | 0.983288 |
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Target: 5'- ---gACCCacgGGACCCacGGGACCa -3' miRNA: 3'- gacaUGGGaaaUCUGGGacCCCUGGc -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 78982 | 0.67 | 0.974784 |
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Target: 5'- -cGUGCCCcu--GAaCCUGGGGGCgCGc -3' miRNA: 3'- gaCAUGGGaaauCUgGGACCCCUG-GC- -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 257372 | 0.67 | 0.969511 |
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Target: 5'- aCUGUACCCUcUAcugaucgugccGACCCUGuGGAaaaaCCGa -3' miRNA: 3'- -GACAUGGGAaAU-----------CUGGGACcCCU----GGC- -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 199765 | 0.69 | 0.931314 |
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Target: 5'- cCUGUGCCCgcucgUUUGGuCCCgaGGGGAUaCGg -3' miRNA: 3'- -GACAUGGG-----AAAUCuGGGa-CCCCUG-GC- -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 85789 | 0.71 | 0.891929 |
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Target: 5'- -cGgcCCCUUaGGACCCUGaGGGCCc -3' miRNA: 3'- gaCauGGGAAaUCUGGGACcCCUGGc -5' |
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| 10320 | 3' | -55.3 | NC_002687.1 | + | 85827 | 1.1 | 0.00671 |
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Target: 5'- aCUGUACCCUUUAGACCCUGGGGACCGg -3' miRNA: 3'- -GACAUGGGAAAUCUGGGACCCCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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