Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10322 | 5' | -52 | NC_002687.1 | + | 282995 | 0.66 | 0.997949 |
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Target: 5'- cGcCG-UGGCUguagcAGCUGUGC-GUGGUg -3' miRNA: 3'- aCuGCaACCGA-----UCGACAUGuCACCAa -5' |
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| 10322 | 5' | -52 | NC_002687.1 | + | 69038 | 0.66 | 0.997135 |
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Target: 5'- uUGGCGaUGGUggcGGCgGUgGCGGUGGUg -3' miRNA: 3'- -ACUGCaACCGa--UCGaCA-UGUCACCAa -5' |
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| 10322 | 5' | -52 | NC_002687.1 | + | 130744 | 0.67 | 0.995429 |
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Target: 5'- gGugGUagcgaUGGUgUAGCUG-GCGGUGGUg -3' miRNA: 3'- aCugCA-----ACCG-AUCGACaUGUCACCAa -5' |
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| 10322 | 5' | -52 | NC_002687.1 | + | 204426 | 0.7 | 0.97252 |
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Target: 5'- cGAUGUUGGCgaagacgaagucccAGCUGUA-GGUGGUa -3' miRNA: 3'- aCUGCAACCGa-------------UCGACAUgUCACCAa -5' |
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| 10322 | 5' | -52 | NC_002687.1 | + | 224178 | 0.71 | 0.946472 |
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Target: 5'- gUGGCGggGGCaguGCUGgugGCGGUGGc- -3' miRNA: 3'- -ACUGCaaCCGau-CGACa--UGUCACCaa -5' |
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| 10322 | 5' | -52 | NC_002687.1 | + | 88640 | 1.05 | 0.02082 |
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Target: 5'- gUGACGUUGGCUAGCUGUACAGUGGUUc -3' miRNA: 3'- -ACUGCAACCGAUCGACAUGUCACCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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