Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10325 | 5' | -59.9 | NC_002687.1 | + | 47465 | 0.66 | 0.914439 |
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Target: 5'- cGGAGCAg-CCuGUAUaugCCGGGGUa -3' miRNA: 3'- aCCUCGUgaGGcCAUAggaGGCCCCA- -5' |
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| 10325 | 5' | -59.9 | NC_002687.1 | + | 217989 | 0.66 | 0.908916 |
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Target: 5'- -uGAGaUACuUUCGGUAcUCUUCCGGGGg -3' miRNA: 3'- acCUC-GUG-AGGCCAU-AGGAGGCCCCa -5' |
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| 10325 | 5' | -59.9 | NC_002687.1 | + | 90005 | 0.66 | 0.884822 |
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Target: 5'- cGGGGguCUCgGGgg-UCUCgGGGGUc -3' miRNA: 3'- aCCUCguGAGgCCauaGGAGgCCCCA- -5' |
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| 10325 | 5' | -59.9 | NC_002687.1 | + | 238216 | 0.67 | 0.857665 |
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Target: 5'- gGGGGC-CUCUGGUGUUCUuuGGa-- -3' miRNA: 3'- aCCUCGuGAGGCCAUAGGAggCCcca -5' |
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| 10325 | 5' | -59.9 | NC_002687.1 | + | 297346 | 0.71 | 0.659624 |
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Target: 5'- cUGG-GCGCUCCGGccccAUCC-CCGGcGGUu -3' miRNA: 3'- -ACCuCGUGAGGCCa---UAGGaGGCC-CCA- -5' |
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| 10325 | 5' | -59.9 | NC_002687.1 | + | 96655 | 1.07 | 0.00444 |
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Target: 5'- aUGGAGCACUCCGGUAUCCUCCGGGGUu -3' miRNA: 3'- -ACCUCGUGAGGCCAUAGGAGGCCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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