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miRNA: 3'- ccaCAGUGGcGGCUGUGCCA-CGGC-CG- -5'
miRNA: 3'- -CCACAGuGGCGGCUgU-GCCACGGCcG- -5'
miRNA: 3'- -CCacagUGGCGGCUGUGCCa---CGGCCG- -5'
miRNA: 3'- -CCACAGUGGCGGCUGUGCCACGGCCG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 10327 | 5' | -59.5 | NC_002687.1 | + | 27192 | 0.76 | 0.43248 |
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Target: 5'- --cGUCGCCcCCGACACGGUcGCUGaGCg -3' miRNA: 3'- ccaCAGUGGcGGCUGUGCCA-CGGC-CG- -5' |
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| 10327 | 5' | -59.5 | NC_002687.1 | + | 89709 | 0.77 | 0.408359 |
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Target: 5'- cGGUGUCuCCGCCGAgGuCGGUGUCGuCg -3' miRNA: 3'- -CCACAGuGGCGGCUgU-GCCACGGCcG- -5' |
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| 10327 | 5' | -59.5 | NC_002687.1 | + | 224848 | 0.8 | 0.282534 |
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Target: 5'- cGGaga-GCCGCCGACGCGGcggcGCCGGCa -3' miRNA: 3'- -CCacagUGGCGGCUGUGCCa---CGGCCG- -5' |
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| 10327 | 5' | -59.5 | NC_002687.1 | + | 97996 | 1.14 | 0.001946 |
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Target: 5'- cGGUGUCACCGCCGACACGGUGCCGGCa -3' miRNA: 3'- -CCACAGUGGCGGCUGUGCCACGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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