miRNA: 3'- ccaCAGUGGCGGCu-------------GUGCCaCG-GCCG- -5'
miRNA: 3'- -CCACa---GUGgCgGCUG-UGCCACGGCCG- -5'
miRNA: 3'- -CCACagugGCGGCUG-------UGCCACGGCCG- -5'
miRNA: 3'- cCACagUGGCGGcUG-UGCC-ACGGCCG- -5'
Results 81 - 84 of 84 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10327 | 5' | -59.5 | NC_002687.1 | + | 307459 | 0.67 | 0.912812 |
|
Target: 5'- --cGUCACCGCCGuccccaucagugucCAUGG-GCgCGGUa -3' miRNA: 3'- ccaCAGUGGCGGCu-------------GUGCCaCG-GCCG- -5' |
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| 10327 | 5' | -59.5 | NC_002687.1 | + | 307628 | 0.73 | 0.619578 |
|
Target: 5'- uGGUGcuggCACgGgCGGCgGCGGUGCUGGUg -3' miRNA: 3'- -CCACa---GUGgCgGCUG-UGCCACGGCCG- -5' |
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| 10327 | 5' | -59.5 | NC_002687.1 | + | 316040 | 0.69 | 0.822816 |
|
Target: 5'- cGGUGg---UGCCGAUggugccgAUGGUGCCGGUg -3' miRNA: 3'- -CCACagugGCGGCUG-------UGCCACGGCCG- -5' |
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| 10327 | 5' | -59.5 | NC_002687.1 | + | 330943 | 0.66 | 0.926208 |
|
Target: 5'- cGUGa-AUCGCC-ACgACGGcUGCCGGCa -3' miRNA: 3'- cCACagUGGCGGcUG-UGCC-ACGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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