Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10331 | 3' | -54.4 | NC_002687.1 | + | 86687 | 0.66 | 0.994267 |
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Target: 5'- cAGA-UCUCGGCAGGaauccCUCCGAUCgUGAa -3' miRNA: 3'- cUCUcAGAGCUGUCU-----GGGGCUAG-ACU- -5' |
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| 10331 | 3' | -54.4 | NC_002687.1 | + | 301723 | 0.66 | 0.992507 |
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Target: 5'- cGAuGGUCUUGGCGucCCCCGGagCUGAa -3' miRNA: 3'- -CUcUCAGAGCUGUcuGGGGCUa-GACU- -5' |
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| 10331 | 3' | -54.4 | NC_002687.1 | + | 8115 | 0.66 | 0.991478 |
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Target: 5'- -----cCUCGACAGACagaCCGGUUUGGu -3' miRNA: 3'- cucucaGAGCUGUCUGg--GGCUAGACU- -5' |
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| 10331 | 3' | -54.4 | NC_002687.1 | + | 236955 | 0.67 | 0.982773 |
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Target: 5'- cGAuAGUCUUGAauucgauGAUUCCGAUCUGAg -3' miRNA: 3'- -CUcUCAGAGCUgu-----CUGGGGCUAGACU- -5' |
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| 10331 | 3' | -54.4 | NC_002687.1 | + | 115994 | 0.69 | 0.96244 |
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Target: 5'- aGAGAGUUUCGAU--ACCCC-AUCUGc -3' miRNA: 3'- -CUCUCAGAGCUGucUGGGGcUAGACu -5' |
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| 10331 | 3' | -54.4 | NC_002687.1 | + | 104884 | 0.76 | 0.667466 |
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Target: 5'- aGAGAGaCUUGACAGACCCUc-UCUGAa -3' miRNA: 3'- -CUCUCaGAGCUGUCUGGGGcuAGACU- -5' |
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| 10331 | 3' | -54.4 | NC_002687.1 | + | 109806 | 1.09 | 0.00956 |
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Target: 5'- cGAGAGUCUCGACAGACCCCGAUCUGAu -3' miRNA: 3'- -CUCUCAGAGCUGUCUGGGGCUAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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