Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10336 | 3' | -53.1 | NC_002687.1 | + | 215279 | 0.66 | 0.99788 |
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Target: 5'- gAGGaaauaUCGgccgGGGuGCAGcgGGUCAaUCGg -3' miRNA: 3'- gUCC-----AGCa---UCCuCGUCuaCCAGU-AGC- -5' |
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| 10336 | 3' | -53.1 | NC_002687.1 | + | 42297 | 0.66 | 0.99788 |
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Target: 5'- aCGGGUUGauguuGGAG-GGAUGGUCgGUCGc -3' miRNA: 3'- -GUCCAGCau---CCUCgUCUACCAG-UAGC- -5' |
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| 10336 | 3' | -53.1 | NC_002687.1 | + | 327467 | 0.66 | 0.996581 |
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Target: 5'- -uGGUCGUAGGGGUcGAUcgcGG-CGUUGg -3' miRNA: 3'- guCCAGCAUCCUCGuCUA---CCaGUAGC- -5' |
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| 10336 | 3' | -53.1 | NC_002687.1 | + | 182226 | 0.66 | 0.996024 |
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Target: 5'- gGGGUCG-AGGGGCgAGuucuUGGaCAUCa -3' miRNA: 3'- gUCCAGCaUCCUCG-UCu---ACCaGUAGc -5' |
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| 10336 | 3' | -53.1 | NC_002687.1 | + | 130758 | 0.69 | 0.979635 |
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Target: 5'- -uGGUCGgAGGAGgGGGUGGUag-CGa -3' miRNA: 3'- guCCAGCaUCCUCgUCUACCAguaGC- -5' |
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| 10336 | 3' | -53.1 | NC_002687.1 | + | 121746 | 1.1 | 0.010886 |
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Target: 5'- cCAGGUCGUAGGAGCAGAUGGUCAUCGg -3' miRNA: 3'- -GUCCAGCAUCCUCGUCUACCAGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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