Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10342 | 3' | -55 | NC_002687.1 | + | 225778 | 0.7 | 0.928924 |
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Target: 5'- uGGAGUAUCguggGGAGgaGGAUGCACCa -3' miRNA: 3'- uCCUCAUGGg---CCUUgaUCUGCGUGGg -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 225964 | 0.7 | 0.928924 |
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Target: 5'- uGGAGUAUCguggGGAGgaGGAUGCACCa -3' miRNA: 3'- uCCUCAUGGg---CCUUgaUCUGCGUGGg -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 232654 | 0.7 | 0.913053 |
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Target: 5'- cGGcGAGUGCCaCGuGAACaUGGACGCggACCUu -3' miRNA: 3'- -UC-CUCAUGG-GC-CUUG-AUCUGCG--UGGG- -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 308802 | 0.71 | 0.901402 |
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Target: 5'- cGGucucaCCGGGAggGGGCGCGCCCg -3' miRNA: 3'- uCCucaugGGCCUUgaUCUGCGUGGG- -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 154877 | 0.72 | 0.860786 |
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Target: 5'- uGGA--ACCCGGAgaggacgcagaagACgcagcAGACGCACCCu -3' miRNA: 3'- uCCUcaUGGGCCU-------------UGa----UCUGCGUGGG- -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 301173 | 0.73 | 0.83113 |
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Target: 5'- uGGGGuUGCCCgGGGACgcaucuuGACGgGCCCa -3' miRNA: 3'- uCCUC-AUGGG-CCUUGau-----CUGCgUGGG- -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 181684 | 0.73 | 0.823111 |
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Target: 5'- -cGAGUGCCgaUGcGAugUGGACGCGCUCa -3' miRNA: 3'- ucCUCAUGG--GC-CUugAUCUGCGUGGG- -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 126256 | 0.76 | 0.678538 |
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Target: 5'- cAGGcucGGUGCCCGGAACauugccGGAUGCACUg -3' miRNA: 3'- -UCC---UCAUGGGCCUUGa-----UCUGCGUGGg -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 120945 | 0.76 | 0.64949 |
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Target: 5'- gGGGuGGUugCUGGGGCUGGuaucCGUACCCg -3' miRNA: 3'- -UCC-UCAugGGCCUUGAUCu---GCGUGGG- -5' |
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| 10342 | 3' | -55 | NC_002687.1 | + | 146920 | 1.13 | 0.005292 |
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Target: 5'- cAGGAGUACCCGGAACUAGACGCACCCa -3' miRNA: 3'- -UCCUCAUGGGCCUUGAUCUGCGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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