Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10343 | 3' | -57.8 | NC_002687.1 | + | 80968 | 0.67 | 0.932097 |
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Target: 5'- aCCACCGccAUGG-C-ACCCuuggACUCGCCUa -3' miRNA: 3'- -GGUGGC--UGCCaGaUGGG----UGAGCGGAg -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 87780 | 0.67 | 0.932097 |
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Target: 5'- gCACCGGCaucugguagccGGgCUGCUgACUCGgCCUCu -3' miRNA: 3'- gGUGGCUG-----------CCaGAUGGgUGAGC-GGAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 75526 | 0.66 | 0.960151 |
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Target: 5'- -gGCuUGACGGaUCUACCCGacCUCGUCaUCg -3' miRNA: 3'- ggUG-GCUGCC-AGAUGGGU--GAGCGG-AG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 139408 | 0.66 | 0.956739 |
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Target: 5'- cCCACUGugGGUaUACCuCGC-CGCaUUCg -3' miRNA: 3'- -GGUGGCugCCAgAUGG-GUGaGCG-GAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 298654 | 0.66 | 0.953132 |
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Target: 5'- aUCAUCGACacuacgcgccuGGUgUGCCCcggCGCCUCu -3' miRNA: 3'- -GGUGGCUG-----------CCAgAUGGGugaGCGGAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 330857 | 0.66 | 0.952387 |
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Target: 5'- aCGCUGACGccacagcagGCCCGCUCGCUg- -3' miRNA: 3'- gGUGGCUGCcaga-----UGGGUGAGCGGag -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 210954 | 0.66 | 0.969263 |
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Target: 5'- aCGCCGAUu-UCUACCgCAUUCGUgUCg -3' miRNA: 3'- gGUGGCUGccAGAUGG-GUGAGCGgAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 25201 | 0.66 | 0.956739 |
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Target: 5'- -gACCGACGcGUUggACCacaaaACUCGCCg- -3' miRNA: 3'- ggUGGCUGC-CAGa-UGGg----UGAGCGGag -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 305215 | 0.66 | 0.963373 |
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Target: 5'- uCCACC-ACcuUCgcgACCCACuucuUCGCCUCg -3' miRNA: 3'- -GGUGGcUGccAGa--UGGGUG----AGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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