Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10343 | 3' | -57.8 | NC_002687.1 | + | 44757 | 0.67 | 0.948544 |
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Target: 5'- -gACCGACGGcCUcaagcacACCCgacuugaccacgaGCUCGCCa- -3' miRNA: 3'- ggUGGCUGCCaGA-------UGGG-------------UGAGCGGag -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 35456 | 0.67 | 0.94112 |
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Target: 5'- aUCACCGcauCGGcagaUGCCCGCcccUGCCUCa -3' miRNA: 3'- -GGUGGCu--GCCag--AUGGGUGa--GCGGAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 110429 | 0.67 | 0.927277 |
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Target: 5'- aCACaCGACa-UCUACgCACUCGCCg- -3' miRNA: 3'- gGUG-GCUGccAGAUGgGUGAGCGGag -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 299371 | 0.68 | 0.91541 |
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Target: 5'- aCCAUCGACccggagacgGGUCUGCUCACUCaaccccgaaguguaGCC-Cg -3' miRNA: 3'- -GGUGGCUG---------CCAGAUGGGUGAG--------------CGGaG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 148141 | 0.69 | 0.881381 |
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Target: 5'- uCCACCGACGGUaaaga-ACUCGUCUg -3' miRNA: 3'- -GGUGGCUGCCAgaugggUGAGCGGAg -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 85728 | 0.69 | 0.860947 |
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Target: 5'- cCCGCUGGCcccgCUGgcCCCGCUgGCCUCg -3' miRNA: 3'- -GGUGGCUGcca-GAU--GGGUGAgCGGAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 308269 | 0.7 | 0.815424 |
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Target: 5'- aCCACCccaGACcaGGUCUcUCCGCgCGCCUCc -3' miRNA: 3'- -GGUGG---CUG--CCAGAuGGGUGaGCGGAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 117618 | 0.74 | 0.606588 |
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Target: 5'- aCACCGugGGUCccaACCCGuucaCGCCUCu -3' miRNA: 3'- gGUGGCugCCAGa--UGGGUga--GCGGAG- -5' |
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| 10343 | 3' | -57.8 | NC_002687.1 | + | 305215 | 0.66 | 0.963373 |
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Target: 5'- uCCACC-ACcuUCgcgACCCACuucuUCGCCUCg -3' miRNA: 3'- -GGUGGcUGccAGa--UGGGUG----AGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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