Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10343 | 5' | -45.3 | NC_002687.1 | + | 281246 | 0.66 | 1 |
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Target: 5'- cAGAAACGGugAGCUgUGACCGc--- -3' miRNA: 3'- uUCUUUGCUcaUCGAaACUGGCaaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 302880 | 0.67 | 1 |
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Target: 5'- aGAGGGAgGGGaaUGGCUgcgggcgcUUGGCCGUUUa -3' miRNA: 3'- -UUCUUUgCUC--AUCGA--------AACUGGCAAAa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 207644 | 0.66 | 1 |
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Target: 5'- gGGGAGACGAGgggAGUgcgcGACCGg--- -3' miRNA: 3'- -UUCUUUGCUCa--UCGaaa-CUGGCaaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 326078 | 0.67 | 0.999999 |
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Target: 5'- uGGGGAUGGGUGuaUUUGACUGUa-- -3' miRNA: 3'- uUCUUUGCUCAUcgAAACUGGCAaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 182330 | 0.67 | 0.999999 |
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Target: 5'- cGGAAGCGAucgcagaUGGCUUUGACCa---- -3' miRNA: 3'- uUCUUUGCUc------AUCGAAACUGGcaaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 214425 | 0.68 | 0.999994 |
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Target: 5'- uGGAAucGCGAGUGGaCggUGACCGa--- -3' miRNA: 3'- uUCUU--UGCUCAUC-GaaACUGGCaaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 89299 | 0.69 | 0.999971 |
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Target: 5'- -cGAAACGAGU-GCg--GACUGUUUa -3' miRNA: 3'- uuCUUUGCUCAuCGaaaCUGGCAAAa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 146670 | 0.71 | 0.999879 |
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Target: 5'- aAAGAGGCGAGUGGgUa-GACCGUc-- -3' miRNA: 3'- -UUCUUUGCUCAUCgAaaCUGGCAaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 145138 | 0.73 | 0.997904 |
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Target: 5'- cAAGGAACaGAcacAGCUUUGACCGUUUUc -3' miRNA: 3'- -UUCUUUG-CUca-UCGAAACUGGCAAAA- -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 300147 | 0.74 | 0.995863 |
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Target: 5'- aAGGGAugGAGUAGCggUGACCc---- -3' miRNA: 3'- -UUCUUugCUCAUCGaaACUGGcaaaa -5' |
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| 10343 | 5' | -45.3 | NC_002687.1 | + | 146999 | 1.03 | 0.119129 |
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Target: 5'- uAAGAAACGAGUAGCUUUGACCGUUUUc -3' miRNA: 3'- -UUCUUUGCUCAUCGAAACUGGCAAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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