Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10349 | 3' | -53.9 | NC_002687.1 | + | 197530 | 0.66 | 0.996402 |
Target: 5'- cCUGGCCgaguguACUGcCUGUGaCGAGUaaGCu -3' miRNA: 3'- -GACCGG------UGGCuGAUACaGCUCAgaCG- -5' |
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10349 | 3' | -53.9 | NC_002687.1 | + | 181619 | 0.66 | 0.995826 |
Target: 5'- gCUGGCUuuGCCGGCUuuuUGcCGGcUUUGCu -3' miRNA: 3'- -GACCGG--UGGCUGAu--ACaGCUcAGACG- -5' |
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10349 | 3' | -53.9 | NC_002687.1 | + | 329715 | 0.66 | 0.994448 |
Target: 5'- cCUGGUCGCCGAaacaGUCGuAGacccgCUGCc -3' miRNA: 3'- -GACCGGUGGCUgauaCAGC-UCa----GACG- -5' |
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10349 | 3' | -53.9 | NC_002687.1 | + | 203677 | 0.66 | 0.992724 |
Target: 5'- --cGUgGCCGACUGUGUCgccccggaagaaGAGUCgaacgGCa -3' miRNA: 3'- gacCGgUGGCUGAUACAG------------CUCAGa----CG- -5' |
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10349 | 3' | -53.9 | NC_002687.1 | + | 55216 | 0.66 | 0.992724 |
Target: 5'- -aGGCCAUCGACUAcUGUUc-GUCgGCc -3' miRNA: 3'- gaCCGGUGGCUGAU-ACAGcuCAGaCG- -5' |
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10349 | 3' | -53.9 | NC_002687.1 | + | 148259 | 0.66 | 0.996402 |
Target: 5'- gUGGCUACCG-CUGgg-CucGUCUGCc -3' miRNA: 3'- gACCGGUGGCuGAUacaGcuCAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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