Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10349 | 3' | -53.9 | NC_002687.1 | + | 59045 | 0.69 | 0.966162 |
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Target: 5'- --aGCCGCCGACacgacgAUGUCGAG-CgacgGCa -3' miRNA: 3'- gacCGGUGGCUGa-----UACAGCUCaGa---CG- -5' |
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| 10349 | 3' | -53.9 | NC_002687.1 | + | 329106 | 0.7 | 0.952296 |
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Target: 5'- uUGGCCACCGAUccu-UgGGGUCUGg -3' miRNA: 3'- gACCGGUGGCUGauacAgCUCAGACg -5' |
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| 10349 | 3' | -53.9 | NC_002687.1 | + | 331665 | 0.7 | 0.952296 |
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Target: 5'- -cGGaCACCGACaGUGUCGGuGUCggugGCu -3' miRNA: 3'- gaCCgGUGGCUGaUACAGCU-CAGa---CG- -5' |
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| 10349 | 3' | -53.9 | NC_002687.1 | + | 75273 | 0.71 | 0.930194 |
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Target: 5'- uUGGuCCuCCGACgugucacugGUGUCGucGUCUGCu -3' miRNA: 3'- gACC-GGuGGCUGa--------UACAGCu-CAGACG- -5' |
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| 10349 | 3' | -53.9 | NC_002687.1 | + | 279082 | 0.71 | 0.925116 |
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Target: 5'- --cGCCAUCGGCUGUGUCuggcggGGGUgUGCc -3' miRNA: 3'- gacCGGUGGCUGAUACAG------CUCAgACG- -5' |
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| 10349 | 3' | -53.9 | NC_002687.1 | + | 167498 | 1.12 | 0.007207 |
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Target: 5'- gCUGGCCACCGACUAUGUCGAGUCUGCu -3' miRNA: 3'- -GACCGGUGGCUGAUACAGCUCAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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