Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10362 | 3' | -64.3 | NC_002687.1 | + | 291083 | 0.69 | 0.53379 |
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Target: 5'- aACC-CGGUcucaCCCCCCcCCCCCUCc -3' miRNA: 3'- cUGGaGCCGcua-GGGGGGuGGGGGAG- -5' |
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| 10362 | 3' | -64.3 | NC_002687.1 | + | 199434 | 0.71 | 0.472919 |
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Target: 5'- -cCCUCGaaggaguCGAUCgCCUCCACCUCCUCg -3' miRNA: 3'- cuGGAGCc------GCUAG-GGGGGUGGGGGAG- -5' |
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| 10362 | 3' | -64.3 | NC_002687.1 | + | 223855 | 0.71 | 0.439739 |
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Target: 5'- cACCaCGGC--UUCCCCCGCCCCCg- -3' miRNA: 3'- cUGGaGCCGcuAGGGGGGUGGGGGag -5' |
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| 10362 | 3' | -64.3 | NC_002687.1 | + | 77630 | 0.75 | 0.283889 |
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Target: 5'- cGACCuguaaUCGGCGcaagucGUCCCCCCACUCCUa- -3' miRNA: 3'- -CUGG-----AGCCGC------UAGGGGGGUGGGGGag -5' |
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| 10362 | 3' | -64.3 | NC_002687.1 | + | 207399 | 0.79 | 0.159799 |
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Target: 5'- cGACCUCGGCGAcCCCCCCGCCg---- -3' miRNA: 3'- -CUGGAGCCGCUaGGGGGGUGGgggag -5' |
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| 10362 | 3' | -64.3 | NC_002687.1 | + | 201417 | 1.09 | 0.00135 |
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Target: 5'- cGACCUCGGCGAUCCCCCCACCCCCUCg -3' miRNA: 3'- -CUGGAGCCGCUAGGGGGGUGGGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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