Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10367 | 3' | -56.2 | NC_002687.1 | + | 263289 | 0.66 | 0.976145 |
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Target: 5'- cGUGCuCGCAUACACCagcuGGAACgaCUGa- -3' miRNA: 3'- uCACG-GUGUGUGUGG----CCUUGg-GACaa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 264054 | 0.72 | 0.740938 |
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Target: 5'- cGGUGCCAuuCACGCACCaGGAACuCCaUGg- -3' miRNA: 3'- -UCACGGU--GUGUGUGG-CCUUG-GG-ACaa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 271195 | 0.67 | 0.946915 |
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Target: 5'- gGGcGCCGCGCACucuugGCCaGAGCUCUGa- -3' miRNA: 3'- -UCaCGGUGUGUG-----UGGcCUUGGGACaa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 291358 | 0.67 | 0.942634 |
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Target: 5'- -uUGCCGCcCGCGCCGGugugcgcgcGCCUUGUc -3' miRNA: 3'- ucACGGUGuGUGUGGCCu--------UGGGACAa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 292717 | 0.67 | 0.958472 |
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Target: 5'- cGUGCgAUACGCAUCGcgcuGAACCCUa-- -3' miRNA: 3'- uCACGgUGUGUGUGGC----CUUGGGAcaa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 295521 | 0.73 | 0.702851 |
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Target: 5'- --cGaCCGCACGCACCGGcGCCgUGUg -3' miRNA: 3'- ucaC-GGUGUGUGUGGCCuUGGgACAa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 296753 | 0.66 | 0.973671 |
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Target: 5'- cGUGuCCAUcgAgGCACCGGAGUCCUGc- -3' miRNA: 3'- uCAC-GGUG--UgUGUGGCCUUGGGACaa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 318074 | 0.69 | 0.874184 |
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Target: 5'- --cGCCGCacaucgcaACACACCGGAACUCUu-- -3' miRNA: 3'- ucaCGGUG--------UGUGUGGCCUUGGGAcaa -5' |
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| 10367 | 3' | -56.2 | NC_002687.1 | + | 318729 | 0.68 | 0.928476 |
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Target: 5'- -cUGUCGCACACGCUGaccACCCUGa- -3' miRNA: 3'- ucACGGUGUGUGUGGCcu-UGGGACaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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