Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1038 | 3' | -51.7 | NC_000924.1 | + | 50825 | 0.66 | 0.900221 |
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Target: 5'- aCGCAUug-AGaag--GUGCGCCcGGACa -3' miRNA: 3'- -GCGUAuuaUCgaagaCACGCGG-CCUG- -5' |
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| 1038 | 3' | -51.7 | NC_000924.1 | + | 59613 | 0.66 | 0.900221 |
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Target: 5'- aGCA--AUGGCUUCaggcGUGCGaCGGAa -3' miRNA: 3'- gCGUauUAUCGAAGa---CACGCgGCCUg -5' |
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| 1038 | 3' | -51.7 | NC_000924.1 | + | 24496 | 0.66 | 0.869703 |
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Target: 5'- aCGCcu-GUGGCUUCUGcaccggaagGCGaugaCGGGCg -3' miRNA: 3'- -GCGuauUAUCGAAGACa--------CGCg---GCCUG- -5' |
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| 1038 | 3' | -51.7 | NC_000924.1 | + | 19331 | 0.66 | 0.869703 |
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Target: 5'- uGCAU--UGGCacau-UGCGCCGGGCa -3' miRNA: 3'- gCGUAuuAUCGaagacACGCGGCCUG- -5' |
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| 1038 | 3' | -51.7 | NC_000924.1 | + | 16545 | 0.67 | 0.825771 |
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Target: 5'- gGCAUGGUcaacGGCUg----GCGCCGGAa -3' miRNA: 3'- gCGUAUUA----UCGAagacaCGCGGCCUg -5' |
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| 1038 | 3' | -51.7 | NC_000924.1 | + | 22440 | 0.7 | 0.701202 |
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Target: 5'- uGCAU--UAGCUUCUGUuaaugcaaugGCGgCGGAUu -3' miRNA: 3'- gCGUAuuAUCGAAGACA----------CGCgGCCUG- -5' |
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| 1038 | 3' | -51.7 | NC_000924.1 | + | 15210 | 1.12 | 0.001522 |
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Target: 5'- aCGCAUAAUAGCUUCUGUGCGCCGGACg -3' miRNA: 3'- -GCGUAUUAUCGAAGACACGCGGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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