Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1038 | 5' | -50.5 | NC_000924.1 | + | 39521 | 0.66 | 0.91148 |
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Target: 5'- --uACcGGUGCGGCAGgc-CCGGCAg -3' miRNA: 3'- gauUGuCCGCGUUGUCauuGGUCGUa -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 27890 | 0.66 | 0.91148 |
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Target: 5'- --cGCAGGCGCuuACG---ACCAGCGg -3' miRNA: 3'- gauUGUCCGCGu-UGUcauUGGUCGUa -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 39394 | 0.66 | 0.904588 |
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Target: 5'- -cAACcGGCG-AACGGgGACCGGCAg -3' miRNA: 3'- gaUUGuCCGCgUUGUCaUUGGUCGUa -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 56213 | 0.66 | 0.897397 |
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Target: 5'- --uGCuGGCGUGACA--AACCGGCAUc -3' miRNA: 3'- gauUGuCCGCGUUGUcaUUGGUCGUA- -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 58261 | 0.67 | 0.882139 |
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Target: 5'- -aAGCAGaGCGUucAACAGUAGCgcaGGCAa -3' miRNA: 3'- gaUUGUC-CGCG--UUGUCAUUGg--UCGUa -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 52163 | 0.68 | 0.829827 |
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Target: 5'- gUGGCAGaguGCGUAACAGUuGCCAGg-- -3' miRNA: 3'- gAUUGUC---CGCGUUGUCAuUGGUCgua -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 38371 | 0.69 | 0.769322 |
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Target: 5'- uCUGACAGuGCaaaGACGGcAACCAGCAg -3' miRNA: 3'- -GAUUGUC-CGcg-UUGUCaUUGGUCGUa -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 49042 | 0.7 | 0.725704 |
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Target: 5'- ----gAGGCGCAACucGGUAACCAGa-- -3' miRNA: 3'- gauugUCCGCGUUG--UCAUUGGUCgua -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 39565 | 0.72 | 0.599734 |
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Target: 5'- -aAACAGGUGCGGCAGgc-CCGGUg- -3' miRNA: 3'- gaUUGUCCGCGUUGUCauuGGUCGua -5' |
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| 1038 | 5' | -50.5 | NC_000924.1 | + | 15245 | 1.07 | 0.003746 |
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Target: 5'- gCUAACAGGCGCAACAGUAACCAGCAUa -3' miRNA: 3'- -GAUUGUCCGCGUUGUCAUUGGUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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