Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10388 | 3' | -52.5 | NC_002687.1 | + | 237752 | 0.7 | 0.963158 |
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Target: 5'- -aGAUCagaGgaaUGCCAGCGACcAUGGCGa -3' miRNA: 3'- uaCUAGg--C---ACGGUUGCUGuUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 316285 | 0.66 | 0.995633 |
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Target: 5'- -gGAUCCG-GUCAugAUGGCGGUGGUg- -3' miRNA: 3'- uaCUAGGCaCGGU--UGCUGUUACCGua -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 170724 | 0.66 | 0.99486 |
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Target: 5'- -cGGUUCGUGCCccgguucGugGGCcgGGUGGCAg -3' miRNA: 3'- uaCUAGGCACGG-------UugCUG--UUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 92877 | 0.67 | 0.992281 |
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Target: 5'- -gGAUCCGUGUCAgucaGCGugGAUGuaGa -3' miRNA: 3'- uaCUAGGCACGGU----UGCugUUACcgUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 21692 | 0.69 | 0.977432 |
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Target: 5'- -aGcgCaugGCCGAUGACAGUGGCGUu -3' miRNA: 3'- uaCuaGgcaCGGUUGCUGUUACCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 163282 | 0.73 | 0.857834 |
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Target: 5'- -gGAUCCGUGUCGAaauCGugAAUGGUGUg -3' miRNA: 3'- uaCUAGGCACGGUU---GCugUUACCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 77730 | 0.73 | 0.850056 |
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Target: 5'- cGUGGUCCGggcacaagGCCAAagaGGCGAUGGCc- -3' miRNA: 3'- -UACUAGGCa-------CGGUUg--CUGUUACCGua -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 48516 | 0.75 | 0.790533 |
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Target: 5'- -aGAUCCGUGCCugUGAUGAUGuGCAc -3' miRNA: 3'- uaCUAGGCACGGuuGCUGUUAC-CGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 239083 | 1.03 | 0.024271 |
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Target: 5'- cAUGAUCCGUGCCAACGACAAUGGCAUc -3' miRNA: 3'- -UACUAGGCACGGUUGCUGUUACCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 182916 | 0.66 | 0.995633 |
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Target: 5'- -cGcUCCGgcaugGCCGugGgACGGUGGCGa -3' miRNA: 3'- uaCuAGGCa----CGGUugC-UGUUACCGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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