Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10388 | 3' | -52.5 | NC_002687.1 | + | 225724 | 0.7 | 0.963158 |
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Target: 5'- -gGGUUgGUGCCGAUGACAacggggAUGGUAg -3' miRNA: 3'- uaCUAGgCACGGUUGCUGU------UACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 237752 | 0.7 | 0.963158 |
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Target: 5'- -aGAUCagaGgaaUGCCAGCGACcAUGGCGa -3' miRNA: 3'- uaCUAGg--C---ACGGUUGCUGuUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 90617 | 0.7 | 0.959682 |
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Target: 5'- --cGUCCuUGCCGACGACGAUacucGGCGUu -3' miRNA: 3'- uacUAGGcACGGUUGCUGUUA----CCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 239836 | 0.72 | 0.918141 |
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Target: 5'- cUGGUCCG-GCCGccGCGGCGGaugcUGGCAa -3' miRNA: 3'- uACUAGGCaCGGU--UGCUGUU----ACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 226801 | 0.73 | 0.879933 |
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Target: 5'- -cGGUgCCgGUGCCggUGGCGGUGGCGg -3' miRNA: 3'- uaCUA-GG-CACGGuuGCUGUUACCGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 163282 | 0.73 | 0.857834 |
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Target: 5'- -gGAUCCGUGUCGAaauCGugAAUGGUGUg -3' miRNA: 3'- uaCUAGGCACGGUU---GCugUUACCGUA- -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 77730 | 0.73 | 0.850056 |
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Target: 5'- cGUGGUCCGggcacaagGCCAAagaGGCGAUGGCc- -3' miRNA: 3'- -UACUAGGCa-------CGGUUg--CUGUUACCGua -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 298615 | 0.74 | 0.833919 |
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Target: 5'- -gGAUCCGUGCgCAuCGACGAUGGa-- -3' miRNA: 3'- uaCUAGGCACG-GUuGCUGUUACCgua -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 48516 | 0.75 | 0.790533 |
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Target: 5'- -aGAUCCGUGCCugUGAUGAUGuGCAc -3' miRNA: 3'- uaCUAGGCACGGuuGCUGUUAC-CGUa -5' |
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| 10388 | 3' | -52.5 | NC_002687.1 | + | 239083 | 1.03 | 0.024271 |
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Target: 5'- cAUGAUCCGUGCCAACGACAAUGGCAUc -3' miRNA: 3'- -UACUAGGCACGGUUGCUGUUACCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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