Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10388 | 5' | -61.8 | NC_002687.1 | + | 123566 | 0.68 | 0.74244 |
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Target: 5'- gUCGCGUGUGGCGaCGGUGucuuguauaCCCACUGc -3' miRNA: 3'- -AGUGCGCGUCGCaGCCACc--------GGGUGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 298416 | 0.69 | 0.669712 |
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Target: 5'- aCGCGCGCGGCGgCGGccgcGGCCUuAUCa -3' miRNA: 3'- aGUGCGCGUCGCaGCCa---CCGGG-UGGc -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 225208 | 0.7 | 0.660437 |
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Target: 5'- -gGCGcCGCcGCGUCGGcGGCUCuCCGa -3' miRNA: 3'- agUGC-GCGuCGCAGCCaCCGGGuGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 295096 | 0.7 | 0.653933 |
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Target: 5'- gCGCGCGCGGgGagcagccaaaugaucUCGGUGGCCguuuuggUACCa -3' miRNA: 3'- aGUGCGCGUCgC---------------AGCCACCGG-------GUGGc -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 215264 | 0.7 | 0.651143 |
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Target: 5'- -gGgGUGCAGCGggucaaUCGGUGGCuCCAUCu -3' miRNA: 3'- agUgCGCGUCGC------AGCCACCG-GGUGGc -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 200587 | 0.71 | 0.586071 |
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Target: 5'- --cCGCGCGGuCGUCGG-GGCCggaaucuuuggCACCGg -3' miRNA: 3'- aguGCGCGUC-GCAGCCaCCGG-----------GUGGC- -5' |
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| 10388 | 5' | -61.8 | NC_002687.1 | + | 297340 | 0.66 | 0.869187 |
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Target: 5'- aCGCGCGCgauGGUGUCGGcGGacugCUugCGg -3' miRNA: 3'- aGUGCGCG---UCGCAGCCaCCg---GGugGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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