Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10390 | 3' | -48 | NC_002687.1 | + | 263704 | 0.7 | 0.999151 |
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Target: 5'- -uGCGugaaUGGCACCGCUCucGACAcGUGGa -3' miRNA: 3'- uuUGUg---ACUGUGGUGAG--UUGUaCACC- -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 252530 | 0.7 | 0.998493 |
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Target: 5'- ---uGCUGACAa-ACUCAACAaGUGGa -3' miRNA: 3'- uuugUGACUGUggUGAGUUGUaCACC- -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 146072 | 0.7 | 0.998493 |
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Target: 5'- aGAAgGaaGACGCCACcguaAGCAUGUGGg -3' miRNA: 3'- -UUUgUgaCUGUGGUGag--UUGUACACC- -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 151664 | 0.71 | 0.998195 |
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Target: 5'- cGAgGCUGGCACCACUCGAuCGUa--- -3' miRNA: 3'- uUUgUGACUGUGGUGAGUU-GUAcacc -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 61181 | 0.71 | 0.997849 |
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Target: 5'- --uCACUGuCGCCGCUCGACGaG-GGa -3' miRNA: 3'- uuuGUGACuGUGGUGAGUUGUaCaCC- -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 9894 | 0.73 | 0.98584 |
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Target: 5'- -cACACUGACGCCggACUUGACGgaugcGUGGc -3' miRNA: 3'- uuUGUGACUGUGG--UGAGUUGUa----CACC- -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 257789 | 0.79 | 0.870709 |
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Target: 5'- aAAACGCUGAUGCCGCaaacCAGCGUGUuGGg -3' miRNA: 3'- -UUUGUGACUGUGGUGa---GUUGUACA-CC- -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 72540 | 0.81 | 0.776936 |
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Target: 5'- -cGCACUGGCACUGCUCGGCgGUGUGu -3' miRNA: 3'- uuUGUGACUGUGGUGAGUUG-UACACc -5' |
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| 10390 | 3' | -48 | NC_002687.1 | + | 241209 | 1.1 | 0.029381 |
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Target: 5'- gAAACACUGACACCACUCAACAUGUGGa -3' miRNA: 3'- -UUUGUGACUGUGGUGAGUUGUACACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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