Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10392 | 3' | -55.7 | NC_002687.1 | + | 78992 | 0.67 | 0.961363 |
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Target: 5'- cGGGGUCcaccgugccCCUGAACCUGGgggcgcGCUGUg -3' miRNA: 3'- -UUCCAGu--------GGACUUGGACCau----CGGCAg -5' |
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| 10392 | 3' | -55.7 | NC_002687.1 | + | 226806 | 0.67 | 0.961363 |
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Target: 5'- -cGGUcCACggGAgcuccagcuGCCUGcGUAGCCGUCu -3' miRNA: 3'- uuCCA-GUGgaCU---------UGGAC-CAUCGGCAG- -5' |
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| 10392 | 3' | -55.7 | NC_002687.1 | + | 87785 | 0.67 | 0.954182 |
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Target: 5'- -cGGUugCACCgGcAUCUGGUAGCCGg- -3' miRNA: 3'- uuCCA--GUGGaCuUGGACCAUCGGCag -5' |
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| 10392 | 3' | -55.7 | NC_002687.1 | + | 139441 | 0.68 | 0.931473 |
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Target: 5'- aAGGGUCAUCaGAAUCUGGaauaaccacucGCCGUCc -3' miRNA: 3'- -UUCCAGUGGaCUUGGACCau---------CGGCAG- -5' |
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| 10392 | 3' | -55.7 | NC_002687.1 | + | 246404 | 1.06 | 0.008938 |
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Target: 5'- aAAGGUCACCUGAACCUGGUAGCCGUCc -3' miRNA: 3'- -UUCCAGUGGACUUGGACCAUCGGCAG- -5' |
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| 10392 | 3' | -55.7 | NC_002687.1 | + | 319511 | 1.07 | 0.00803 |
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Target: 5'- aAAGGUCACCUGAACCUGGUAGCCGUCu -3' miRNA: 3'- -UUCCAGUGGACUUGGACCAUCGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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