Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10409 | 5' | -57.4 | NC_002687.1 | + | 284649 | 0.73 | 0.612769 |
Target: 5'- gGUGCUGCCGGAaaaaaGACCCACCAAg -3' miRNA: 3'- -UACGACGGCUUcccugUUGGGUGGUU- -5' |
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10409 | 5' | -57.4 | NC_002687.1 | + | 296948 | 0.67 | 0.912081 |
Target: 5'- cUGCUGCacCGggGGGugAGCUUGCuCGAc -3' miRNA: 3'- uACGACG--GCuuCCCugUUGGGUG-GUU- -5' |
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10409 | 5' | -57.4 | NC_002687.1 | + | 301176 | 0.66 | 0.946862 |
Target: 5'- -gGUUGCCc-GGGGACGcaucuugacggGCCCAUCAAc -3' miRNA: 3'- uaCGACGGcuUCCCUGU-----------UGGGUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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