Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10414 | 5' | -52.4 | NC_002687.1 | + | 150950 | 0.66 | 0.996051 |
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Target: 5'- aAGAacagGGCGGGaaacuacugGGCGCACGAc- -3' miRNA: 3'- -UCUa---CCGCCUcaaaa----CCGCGUGCUuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 224320 | 0.66 | 0.996228 |
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Target: 5'- uGAUGGCGGuggcagugcugguGGUggUGGCGaugGCGAu- -3' miRNA: 3'- uCUACCGCC-------------UCAaaACCGCg--UGCUuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 238451 | 0.66 | 0.996286 |
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Target: 5'- uGGcAUGGaaGGAGUUcaGGUGCugGAGAa -3' miRNA: 3'- -UC-UACCg-CCUCAAaaCCGCGugCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 274423 | 0.66 | 0.996286 |
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Target: 5'- -cGUGGCGGuuGUUcUGaCGCACGGAGg -3' miRNA: 3'- ucUACCGCCu-CAAaACcGCGUGCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 130721 | 0.66 | 0.997294 |
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Target: 5'- cGGUGGUGGuGGUggcggUGGCGgugGCGAAAg -3' miRNA: 3'- uCUACCGCC-UCAaa---ACCGCg--UGCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 173592 | 0.66 | 0.997706 |
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Target: 5'- cGGA-GGCGGAGgcgggGGCGgagGCGGAGg -3' miRNA: 3'- -UCUaCCGCCUCaaaa-CCGCg--UGCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 325101 | 0.66 | 0.998064 |
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Target: 5'- uAGAUGGCGGAucuugGUUUUuGCGCucuUGAc- -3' miRNA: 3'- -UCUACCGCCU-----CAAAAcCGCGu--GCUuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 323950 | 0.66 | 0.998064 |
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Target: 5'- uAGAUGGCGGAucuugGUUUUuGCGCucuUGAc- -3' miRNA: 3'- -UCUACCGCCU-----CAAAAcCGCGu--GCUuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 89059 | 0.66 | 0.998064 |
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Target: 5'- uGGUGGUGGuGGUggUGGUGUcugucGCGggGg -3' miRNA: 3'- uCUACCGCC-UCAaaACCGCG-----UGCuuU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 173622 | 0.66 | 0.998064 |
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Target: 5'- cGGA-GGCGGGGgcggaGGCGgaCGCGGAAg -3' miRNA: 3'- -UCUaCCGCCUCaaaa-CCGC--GUGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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