Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10414 | 5' | -52.4 | NC_002687.1 | + | 224082 | 0.69 | 0.979989 |
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Target: 5'- cGGAUGGUGGuGGUgcUGGCGgACGu-- -3' miRNA: 3'- -UCUACCGCC-UCAaaACCGCgUGCuuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 307640 | 0.69 | 0.977745 |
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Target: 5'- cGGUGGCGGuGg--UGGUGCugGc-- -3' miRNA: 3'- uCUACCGCCuCaaaACCGCGugCuuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 225020 | 0.69 | 0.972711 |
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Target: 5'- cGGGUGGUGGcGUg--GGCGUcCGAGAc -3' miRNA: 3'- -UCUACCGCCuCAaaaCCGCGuGCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 224140 | 0.7 | 0.960267 |
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Target: 5'- cGGUGGCGGuGgcgcUGGCGCugGc-- -3' miRNA: 3'- uCUACCGCCuCaaa-ACCGCGugCuuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 224116 | 0.7 | 0.960267 |
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Target: 5'- cGGUGGCGGuGgcgcUGGCGCugGc-- -3' miRNA: 3'- uCUACCGCCuCaaa-ACCGCGugCuuu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 292787 | 0.71 | 0.930049 |
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Target: 5'- cGGUGGCGGccgc--GGCGCACGGGc -3' miRNA: 3'- uCUACCGCCucaaaaCCGCGUGCUUu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 154217 | 0.76 | 0.727406 |
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Target: 5'- gGGGUGGCGu-GUUccccUGGCGCACGAAGa -3' miRNA: 3'- -UCUACCGCcuCAAa---ACCGCGUGCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 292673 | 0.78 | 0.667722 |
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Target: 5'- -uGUGGCGGuGGUUUUGG-GCACGAAAg -3' miRNA: 3'- ucUACCGCC-UCAAAACCgCGUGCUUU- -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 211940 | 0.79 | 0.566785 |
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Target: 5'- uGAUGGUGGAGacggaGGCGCACGGAu -3' miRNA: 3'- uCUACCGCCUCaaaa-CCGCGUGCUUu -5' |
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| 10414 | 5' | -52.4 | NC_002687.1 | + | 295550 | 1.07 | 0.016606 |
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Target: 5'- aAGAUGGCGGAGUUUUGGCGCACGAAAg -3' miRNA: 3'- -UCUACCGCCUCAAAACCGCGUGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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